Glyceride composition of processed fats and oils as determined by glass capillary gas chromatography

  • R. P. D’Alonzo
  • W. J. Kozarek
  • R. L. Wade


Refined and bleached fats and oils can be analyzed directly by high-temperature glass capillary column gas chromatography after derivatization of the fatty acids, mono- and diglycerides with BSTFA [(N,O)-bis(trimethyl silyl) trifluoroacetamide]. The intact glycerides are separated on the basis of volatility to provide characteristic carbon number profiles (CNP). Quantitative information on mono-, di- and triglycerides, as well as free fatty acids, is obtained from a single, rapid separation. Profile values are reported for 13 different processed fats and oils. The analysis performed in the split-injection mode maintains column integrity after numerous separations, while producing acceptable relative standard deviations.


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Copyright information

© the American Oil chemists’ Society 1982

Authors and Affiliations

  • R. P. D’Alonzo
    • 1
  • W. J. Kozarek
    • 1
  • R. L. Wade
    • 1
  1. 1.The Procter & Gamble CompanyCincinnati

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