The effect of nonionic surfactant structure on hemolysis

  • Masako Ohnishi
  • Hiromichi Sagitani


The hemolytic properties of polyoxyethylene-type nonionic surfactants were investigated in the concentration range of 0.1–5.0 wt/vol%. The effects of hydrophiliclipophilic balance, molecular weight, chemical structure and solubilizing ability of the nonionic surfactants in hemolysis are discussed. Bulky surfactants with three or four alkyl chains in their molecules, which show low solubilizing ability of lipids, were the least likely to induce hemolysis. Morphological observations of erythrocytes by video-enhanced microscopy showed that linear surfactants induce the formation of spherocytes and cause hemolysis, whereas bulky surfactants induce only morphological changes from discocytes to spheroechinocytes.

Key words

Bulky structure hemolysis morphological change nonionic surfactant 


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Copyright information

© The American Oil Chemists’ Society 1993

Authors and Affiliations

  • Masako Ohnishi
    • 1
  • Hiromichi Sagitani
    • 1
  1. 1.Pola Chemical Industries Inc.Yokohama Research CenterYokohamaJapan

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