In Vitro

, Volume 17, Issue 6, pp 519–530 | Cite as

High density lipoproteins and the growth of vascular endothelial cells in serum-free medium

  • J.-P. -P. Tauber
  • J. Cheng
  • S. Massoglia
  • D. Gospodarowicz


Low density bovine vacular endothelial cell cultures maintained on dishes coated with an extracellular matrix can be grown in serum-free Dulbecco's modified Eagle's medium supplemented with high density lipoprotein (HDL) and transferrin. Such cultures do not require insulin. Early passage cultures exposed to HDL and transferrin grew as well as cultures exposed to optimal serum concentrations and could be passaged repeatedly in total absence of serum. A requirement for fibroblast growth factor to ensure an optimal growth could be observed only with late-passage cultures. The present results suggest strongly that HDL is involved in supporting the proliferation of vascular endothelial cells in vitro. This may be important for our understanding of the biological role of HDL “in vivo”.

Key words

HDL growth control vascular endothelium extracellular matrix 


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Copyright information

© Tissue Culture Association, Inc 1981

Authors and Affiliations

  • J.-P. -P. Tauber
    • 1
    • 2
  • J. Cheng
    • 1
    • 2
  • S. Massoglia
    • 1
    • 2
  • D. Gospodarowicz
    • 1
    • 2
  1. 1.Cancer Research InstituteUniversity of California Medical CenterSan Francisco
  2. 2.Departments of Medicine and OphthalmologyUniversity of California Medical CenterSan Francisco

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