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In Vitro

, Volume 17, Issue 6, pp 519–530 | Cite as

High density lipoproteins and the growth of vascular endothelial cells in serum-free medium

  • J.-P. -P. Tauber
  • J. Cheng
  • S. Massoglia
  • D. Gospodarowicz
Article

Summary

Low density bovine vacular endothelial cell cultures maintained on dishes coated with an extracellular matrix can be grown in serum-free Dulbecco's modified Eagle's medium supplemented with high density lipoprotein (HDL) and transferrin. Such cultures do not require insulin. Early passage cultures exposed to HDL and transferrin grew as well as cultures exposed to optimal serum concentrations and could be passaged repeatedly in total absence of serum. A requirement for fibroblast growth factor to ensure an optimal growth could be observed only with late-passage cultures. The present results suggest strongly that HDL is involved in supporting the proliferation of vascular endothelial cells in vitro. This may be important for our understanding of the biological role of HDL “in vivo”.

Key words

HDL growth control vascular endothelium extracellular matrix 

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References

  1. 1.
    Gospodarowicz, D.; Ill, C. R. The extracellular matrix and the control of proliferation of vascular endothelial cells. J. Clin. Invest. 65: 1351–1364; 1980.PubMedGoogle Scholar
  2. 2.
    Gospodarowicz, D.; Savion, N.; Vlodavsky, I. The extracellular matrix and the control of proliferation of vascular endothelial and vascular smooth muscle cells. J. Supramolecular Struct. 13: 339–372; 1980.CrossRefGoogle Scholar
  3. 3.
    Gospodarowicz, D.; Delgado, D.; Vlodavsky, I. Permissive effect of the extracellular matrix on cell proliferation in vitro. Proc. Natl. Acad. Sci. USA 77: 4094–4098; 1980.PubMedCrossRefGoogle Scholar
  4. 4.
    Miller, G. J.; Miller, N. E. Plasma-high-density-lipoprotein concentration and development of ischemic heart disease. Lancet 1: 16–19; 1975.PubMedCrossRefGoogle Scholar
  5. 5.
    Tauber, J.-P.; Cheng, J.; Gospodarowicz, D. The effect of high and low density lipoproteins on the proliferation of vascular endothelial cells maintained in the presence of lipoprotein-deficient serum. J. Clin. Invest. 66: 696–708; 1980.PubMedGoogle Scholar
  6. 6.
    Gospodarowicz, D.; Bialecki, H.; Greenburg, G. Purification of the fibroblast growth factor activity from bovine brain. J. Biol. Chem. 253: 3736–3743; 1978.PubMedGoogle Scholar
  7. 7.
    Havel, R. J.; Eder, H. S.; Bragdon, J. A. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. Clin. Invest. 34: 1345–1353; 1955.PubMedGoogle Scholar
  8. 8.
    Jonas, A. Physicochemical properties of bovine serum high density lipoproteins. J. Biol. Chem. 247: 7767–7772; 1972.PubMedGoogle Scholar
  9. 9.
    Gospodarowicz, D.; Greenburg, G. The coating of bovine and rabbit corneas denuded of their endothelium with bovine corneal endothelial cells. Exp. Eye Res. 38: 249–265; 1978.Google Scholar
  10. 10.
    Gospodarowicz, D.; Mescher, A. L.; Birdwell, C. R. Stimulation of corneal endothelial cells in vitro by fibroblast and epidermal growth factors. Exp. Eye Res. 25: 75–87; 1977.PubMedCrossRefGoogle Scholar
  11. 11.
    Gospodarowicz, D.; Hirabayashi, K.; Giguere, L.; Tauber, J.-P. Factors controlling the proliferative rate, final cell density, and life span of bovine vascular smooth muscle cells in culture. J. Cell Biol. In press.Google Scholar
  12. 12.
    Gospodarowicz, D.; Greenburg, G.; Bialecki, H.; Zetter, B. Factors involved in the modulation of cell proliferation in vivo and in vitro: The role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells. In Vitro 14: 85–118; 1978.PubMedCrossRefGoogle Scholar
  13. 13.
    Vlodavsky, I.; Johnson, L. K.; Greenburg, G.; Gospodarowicz, D. Vascular endothelial cells maintained in the absence of fibroblast growth factor undergo structural and functional alterations that are incompatible with their in vivo differentiated properties. J. Cell Biol. 83: 468–483; 1979.PubMedCrossRefGoogle Scholar
  14. 14.
    Phillips, J. L. Specific binding of radioiodinated transferrin to polypropylene culture tubes. Biochem. Biophys. Res. Commun. 71: 726–732; 1976.PubMedCrossRefGoogle Scholar
  15. 15.
    Barnes, D.; Sato, G. Serum-free cell culture: a unifying approach. Cell 22: 649–655; 1980.PubMedCrossRefGoogle Scholar
  16. 16.
    Bottenstein, J.; Hayashi, I.; Hutchings, S.; Masui, H.; Mather, J.; McClure, D.; Ohasa, S.; Rizzino, A.; Sato, G.; Serrero, G.; Wolfe, R.; Wu, R. The growth of cells in serum-free hormone-supplemented medium. Methods Enzymol. 58: 94–109; 1978.CrossRefGoogle Scholar
  17. 17.
    Sato, G. The role of serum in cell culture. Litwak, G. ed. Biochemical action of hormones. Vol. 3. New York: Academic Press; 1975: 391–396.Google Scholar
  18. 18.
    Sato, G. H.; Reid, L. Replacement of serum in cell culture by hormones. Biochemistry and mode of action of hormones II. Rickenberg, H. V. ed. Vol. 20. Baltimore, MD: University Park; 1978: 215–256.Google Scholar
  19. 19.
    Hutchings, S. E.; Sato, G. H. Growth and maintenance of Hela cells in serum-free medium supplemented with hormones. Proc. Natl. Acad. Sci. USA 75: 901–904; 1978.PubMedCrossRefGoogle Scholar
  20. 20.
    Rizzino, A.; Sato, G. Growth of embryonal carcinoma cells in serum-free medium. Proc. Natl. Acad. Sci. USA 75: 1844–1848; 1978.PubMedCrossRefGoogle Scholar
  21. 21.
    Gospodarowicz, D.; Fujii, D. K. The extracellular matrix and the control of cell proliferation and differentiation. Scott, W. A.; Werner, R.; Schultz, J. eds. Cellular responses to molecular modulators. Miami Winter Symposia, Vol. 18. In press.Google Scholar
  22. 22.
    Savion, N.; Liu, G. M.; Laherty, R.; Gospodarowicz, D. Factors controlling the proliferation and progesterone production by bovine granulosa cells in serum free medium. Endocrinology. In press.Google Scholar

Copyright information

© Tissue Culture Association, Inc 1981

Authors and Affiliations

  • J.-P. -P. Tauber
    • 1
    • 2
  • J. Cheng
    • 1
    • 2
  • S. Massoglia
    • 1
    • 2
  • D. Gospodarowicz
    • 1
    • 2
  1. 1.Cancer Research InstituteUniversity of California Medical CenterSan Francisco
  2. 2.Departments of Medicine and OphthalmologyUniversity of California Medical CenterSan Francisco

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