Advertisement

Tropical Animal Health and Production

, Volume 29, Supplement 4, pp 119S–123S | Cite as

Control ofTheileria sergenti infection by vaccination

  • M. Onuma
  • S. Kubota
  • T. Kakuda
  • Y. Sako
  • M. Asada
  • H. Kabeya
  • C. Sugimoto
Vaccination

Abstract

Bovine piroplasmosis caused byTheileria sergenti is a major cause of economic loss in grazing cattle in Japan. Infected calves show chronic anaemia with intraerythrocytic piroplasms and occasionally die in severe cases. We found that parasite stocks and isolates consist of genetically and antigenically mixed populations. To differentiate parasite populations bearing 3 allelic forms of p32/34, an immunodominant piroplasm surface protein, 3 sets of oligonucleotide primers were designed to amplify either of 3 alleles by polymerase chain reaction (PCR). By using this allele-specific PCR, we found that the majority ofT. sergenti-infected calves in Japan harbored mixed parasite populations bearing C and I type parasites. To controlTheileria infection, we produced 2 vaccine candidates: recombinant baculovirus p32 and synthetic peptide containing Lys-Glu-Lys (KEK) motif. Immunization with either recombinant p32 or synthetic peptide containing KEK sequences with Freund’s complete adjuvant resulted in low parasitemia and reduced the clinical symptoms compared to control calves. Interestingly, the parasite with the p32 allelic form corresponding to the one used as the immunogen was suppressed.

Keywords

Bovine Leukemia Virus Parasite Population Veterinary Parasitology Control Calf Type Parasite 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Kubota S., Sugimoto C. &Onuma M. (1996a). Population dynamics ofTheileria sergenti infected cattle and vector ticks analysed by a PCR.Parasitology,112, 437–442.PubMedGoogle Scholar
  2. Kubota S., Sugimoto C., Kakuda T. &Onuma M. (1996b). Parasite population analysis ofTheileria sergenti andT. buffeli by allele-specific polymerase chain reaction for immunodominant piroplasm surface antigen genes.International Journal for Parasitology,26, 741–748.PubMedCrossRefGoogle Scholar
  3. Kubota S., Sugimoto C. &Onuma M. (1995). A genetic analysis of mixed population inTheileria sergenti stocks and isolates using allele-specific polymerase chain reaction.Journal of Veterinary Medicine and Science,57, 279–282.Google Scholar
  4. Molano A., Segura C., Guzman F., Lozada D. &Patarroyo M. E. (1991). In human malaria protective antibodies are directed mainly against the Lys-Glu ion pair within the Lys-Glu-Lys motif of the synthetic vaccine SPf 66.Parasite Immunology,14, 111–124.Google Scholar
  5. Matsuba T., Kawakami Y., Jwai H. &Onuma M. (1992). Genomic analysis ofTheleria sergenti stocks in Japan with DNA probes.Veterinary Parasitology,41, 35–43.PubMedCrossRefGoogle Scholar
  6. Matsuba T., Kubota H., Tanaka M., Hattori M., Sugimoto C. &Onuma M. (1993a). Analysis of a mixed parasite population by using cDNA probes encoding a major piroplasm surface protein ofTheileria sergenti.Parasitology,107, 369–377.PubMedCrossRefGoogle Scholar
  7. Matsuba T., Sugimoto C., Hattori M., Sako Y., Fujisaki K. &Onuma M. (1995). Expression of a 32KDTheileria sergenti piroplasm surface protein by recombinant baculoviruses.International Journal of Parasitology,25, 939–943.PubMedCrossRefGoogle Scholar
  8. Matsuba T., Sugimoto C., Onoe S., Kawakami Y., Iwai H. andOnuma M. (1993b). Changes in the hybridization patterns of populations ofTheileria sergenti during infection.Veterinary Parasitology,47, 215–223.PubMedCrossRefGoogle Scholar
  9. Ohishi K., Kabeya H., Amanuma H. &Onuma M. Induction of bovine leukemia virus Env-specific Th1 type immunity in mice by vaccination with short synthesized peptide-liposome.Vaccine, In press.Google Scholar
  10. Shirakata S., Onuma M., Kirisawa R., Takahashi K. &Kawakami Y. (1989). Localization of surface antigen onTheileria sergenti merozoite by monoclonal antibodies.Japanese Journal of Veterinary Science 51, 831–833.PubMedGoogle Scholar
  11. Tanaka M., Ohgitani T., Kawamoto S., Takahashi K., Onuma M., Kawakami Y. &Sasaki N. (1990). Protective effect against intraerythrocytic merozoites ofTheileria sergenti infection in calves by passive transfer of monoclonal antibody.Japanese Journal of Veterinary Science,52, 631–633.PubMedGoogle Scholar
  12. Tanaka M., Onoe S., Matsuba T., Katayama S., Yamanaka M., Yonemichi H., Hiramatsu K., Baek B. K., Sugimoto C. &Onuma M. (1993). Detection ofTheileria sergenti infection in cattle by polymerase chain reaction amplification of parasite-specific DNA.Journal of Clinical Microbiology,31, 2565–2569.PubMedGoogle Scholar
  13. Zhuang W. Z., Sugimoto C. et al. (1996). Antigenic alteration in major piroplasm surface proteins ofTheileria sergenti during infection.Veterinary Parasitology,60, 191–198.CrossRefGoogle Scholar
  14. Zhuang W. Z., Sugimoto C., Matsuba T., Niinuma S., Murata M. &Onuma M. (1994). Analyses of antigenic and genetic diversities ofTheileria sergenti piroplasm surface proteins.Journal Veterinary Medicine & Science 56, 469–473.Google Scholar

Copyright information

© Centre for Tropical Veterinary Medicine 1997

Authors and Affiliations

  • M. Onuma
    • 1
  • S. Kubota
    • 1
  • T. Kakuda
    • 1
  • Y. Sako
    • 1
  • M. Asada
    • 1
  • H. Kabeya
    • 1
  • C. Sugimoto
    • 1
  1. 1.Department of Disease Control, Graduate School of Veterinary MedicineHokkaido UniversitySapporoJapan

Personalised recommendations