The composition of beeswax alkyl esters

  • P. J. Holloway
Technical

Abstract

The alkyl esters of beeswax, after isolation from the unhydrolyzed wax by preparative layer chromatography (PLC), have been analyzed directly by high temperature GLC using 1.5% OV1 as liquid phase. In two commercial wax samples examined the ester homologues are predominantly even carbon numbered ranging from C36 to C54. The principal alkyl esters are C40, C42, C44, C46 and C48. The GLC analysis of the ester hydrolysis products revealed that the variations in ester chain length are produced by variations in the esterified primary alcohol chain lengths. The esterified fatty acid is chiefly hexadecanoic acid. The esterified fatty acids differ in composition from the free fatty acids which are also present in the wax.

References

  1. 1.
    Downing, D. T., Z. H. Kranz, J. A. Lamberton, K. E. Murray and A. H. Redcliffe, Aust. J. Chem.14, 253–263 (1961).CrossRefGoogle Scholar
  2. 2.
    Toyama, Y., and H. Hirai, Fette u. Seif.53, 556–557 (1951).CrossRefGoogle Scholar
  3. 3.
    Streibl, M., K. Stransky and F. Sorm, Fette, Seifen, Anstrichmittel68, 799–805 (1966).Google Scholar
  4. 4.
    Stransky, K., M. Streibl and F. Šorm, Collect, Czech. Chem. Commun. Engl. Ed31, 4694–4702 (1966).Google Scholar
  5. 5.
    Warth, A. H., “The Chemistry and Technology of Waxes,” 2nd Ed., Reinhold, New York, 1956, p. 76.Google Scholar
  6. 6.
    Kaufmann, H. P., and B. Das, Fette, Seifen, Anstrichmittel65, 398–402 (1963).CrossRefGoogle Scholar
  7. 7.
    Hessler, W., and F. Sammet, Ibid.67, 552–557 (1965).CrossRefGoogle Scholar
  8. 8.
    Holloway, P. J., and S. B. Challen, Chromatog.25, 336–346 (1966).CrossRefGoogle Scholar
  9. 9.
    Callow, R. K., Bee World44, 95–101 (1963).Google Scholar
  10. 10.
    Toyama, Y., and H. Hirai, Res. Rep. Nagoya Industr. Sci. Res. Inst. No. 3, 42–45 (1951).Google Scholar
  11. 11.
    Holloway, P. J., J. Pharm. Pharmacol.29, 775–779 (1968).Google Scholar
  12. 12.
    Goddu, R. F., N. F. Leblanc and C. M. Wright, Anal. Chem.27, 1251–1255 (1955).CrossRefGoogle Scholar
  13. 13.
    Mazliak, P., Phytochemistry2, 253–261 (1963).CrossRefGoogle Scholar
  14. 14.
    Holloway, P. J., Ph.D. Thesis. University of London (1967).Google Scholar
  15. 15.
    Ludwig, F. J., Soap Chem. Spec.43, 70–82 (1966).Google Scholar
  16. 16.
    Radler, F., Aust. J. Biol. Sci.18, 1045–1056 (1965).Google Scholar

Copyright information

© The American Oil Chemists’ Society 1969

Authors and Affiliations

  • P. J. Holloway
    • 1
  1. 1.Long Ashton Research StationUniversity of BristolEngland

Personalised recommendations