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In vitro shoot multiplication of eastern white cedar (Thuja occidentalis)

Summary

A protocol for clonal propagation of eastern white cedar (Thuja occidentalis L.) was enhanced by optimizing the shoot multiplication stage using unbranched in vitro-produced shoots. This was achieved by careful selection of different medium components. An optimum range of 10 to 14 axillary shoots was obtained when shoots were cultured on half-strength Quiorin and LePoivre medium containing 10µM filter-sterilized zeatin for 3 wk. Transfer of the treated shoots to cytokinin-free medium containing 0.05% activated charcoal improved both the number and quality of the axillary shoots produced. Maximum axillary bud induction was also accomplished when shoots were pulsed in 1 mM liquid, filter-sterilized zeatin for 3 h, and then transferred to half-strength Quiorin and LePoivre, charcoal-containing medium. Inclusion of 4% sucrose improved the number of axillary shoots obtained. Half strength of the major salts produced an optimum response. Shoots obtained from different cultures (1 to 5 yr old) responded similarly to the applied cytokinin; however, newly induced shoots (4 mo. old) gave a significantly higher response.

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References

  1. Baxter, R.; Brown, S. N.; England, N. F., et al. Production of clonal plantlets of tropical pine in tissue culture via axillary shoot activation. Can. J. For. Res. 19:1338–1342; 1989.

  2. Biondi, S.; Thorpe, T. A. Clonal propagation of forest tree species. In: Rao, A. N., ed. Tissue culture of economically important plants. Singapore: COSTED and Asian Network for Biological Sciences; 1982:197–204.

  3. Brock, T. G.; Kaufman, P. B. Growth regulators: an account of hormones and growth regulation. In: Stewart, F. C., ed. Growth and development. Vol. X. Plant physiology, a treatise. New York: Academic Press; 1991:277–338.

  4. Burns, J. A.; Schwarz, O. J.; Schlarbaum, S. E. Multiple shoot production from seedling explants of slash pine (Pinus elliottii, Engelm.). Plant Cell Rep. 10:439–443; 1991.

  5. David, A. In vitro propagation of gymnosperms. In: Bonga, J. M.; Durzan, D. J., eds. Tissue culture in forestry. The Hague: Martinus Nijhoff; 1982:72–108.

  6. Dunstan, D. I.; Lashta, D. P.; Kikcio, S., et al. Factors affecting recurrent shoot multiplication on in vitro cultures of 17- to 20-year-old Douglas fir trees. In Vitro Cell. Dev. Biol. 28P:33–38; 1992.

  7. Durzan, D. J.; Campbell, R. A. Prospects for the mass production of improved stock of forest trees by cell and tissue culture. Can. J. For. Res. 4:151–160; 1974.

  8. Fellman, C. D.; Read, P. E.; Hosier, M. A. Effect of thidiazuron and CPPU on meristem formation and shoot proliferation. Chemical regulation in tissue culture: an overview. HortScience 22:1197–1200; 1987.

  9. Goldfarb, B.; Howe, G. T.; Bailey, L. M., et al. A liquid cytokinin pulse induces adventitious shoot formation from Douglas-fir cotyledons. Plant Cell Rep. 10:156–160; 1991.

  10. Gray, D. J.; Benton, C. M. In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundiflia). Plant Cell Tissue Organ Cult. 27:7–14; 1991.

  11. Halperin, W. Attainment and retention of morphogenic capacity in vitro. In: Vasil, I. K., ed. Cell culture and somatic cell genetics of plants, vol. 3. New York: Academic Press; 1986:3–47.

  12. Harry, I. S.; Thompson, M. R.; Thorpe, T. A. Regeneration of plantlets from mature embryos of western larch. In Vitro Cell. Dev. Biol. 27P:89–98; 1991.

  13. Harry, I. S.; Thompson, M. R.; Lu, C-Y., et al. In vitro plantlet formation from embryonic explants of eastern white cedar (Thuja occidentalis L.). Tree Physiol. 3:273–283; 1987.

  14. Hosie, R. C. Native trees of Canada, 7th ed. Don Mills, Ont. Canada: Fitzhenry and Whiteside Ltd.; 1979:96–99.

  15. Lu, C-Y.; Harry, I. S.; Thompson, M. R., et al. Plantlet regeneration from cultured embryos and seedling parts of red spruce (Picea rubens Sarg.). Bot. Gaz. 152:42–50; 1991.

  16. Martinez Pulido, C.; Harry, I. S.; Thorpe, T. A. In vitro regeneration of plantlets of Canary Island pine (Pinus canariensis). Can. J. For. Res. 20:1200–1211; 1990.

  17. Mok, M. C.; Mok, D. W. S.; Armstrong, D. J., et al. Cytokinin activity ofN-phenyl-N’-1,2,3-thiadiazol-5-ylurea. Phytochemistry 21:1509–1511; 1982.

  18. Mok, M. C.; Mok, D. W. S.; Turner, J. E., et al. Biological and biochemical effect of cytokinin-active phenylurea derivatives in tissue culture systems. Proceedings of the Symposium on Chemical Regulation in Tissue Culture: An overview. HortScience 22:1192–1194; 1987.

  19. Patel, K. R.; Kim, H-R.; Thorpe, T. A. Plantlet formation in pitch pine (Pinus rigida Mill.) by tissue culture methods. For. Ecol. Manage. 15:147–160; 1986.

  20. Phillips, I. D. J. Apical dominance. Annu. Rev. Plant Physiol. 26:341–367; 1975.

  21. Quoirin, M.; LePoivre, P. Etude de milieux adaptes aux cultures in vitro de prunus. Acta Hortic. 78:437–442; 1977.

  22. Rumary, C.; Thorpe, T. A. Plantlet formation in black and white spruce. I. In vitro techniques. Can J. For. Res. 14:10–16; 1984.

  23. SAS Institute. SAS user’s guide: statistics version 6 ed. Cary, NC: SAS Institute; 1985.

  24. Tamas, I. A. Hormonal regulation of apical dominance. In: Davies, P. J., ed. Plant hormones and their role in plant growth and development. Dordrecht: Martinus Nijhoff Publishers; 1987:393–410.

  25. Thorpe, T. A.; Harry, I. S.; Kumar, P. P. Application of micropropagation to forestry. In: Debergh, P. C.; Zimmerman, R. H., eds. Micropropagation, technology and application. Dordrecht: Kluwer Academic Publishers; 1991:311–336.

  26. van Gelderen, D. M.; van Hoey Smith, J. R. P. Conifers, 2nd ed. Oregon: Timber Press; 1989:27–28.

  27. van Nieuwkerk, J. P.; Zimmerman, R. H.; Fordham, I. Thidiazuron stimulation of apple shoot proliferation in vitro. HortScience 21:516–518; 1986.

  28. Vogelmann, T. C.; Bornman, C. H.; Nissen, P. Uptake of benzyladenine in explants ofPicea abies andPinus sylvestris. Physiol. Plant. 61:513–517; 1984.

  29. Zel, J.; Gogala, N.; Camloh, M. Micropropagation ofPinus sylvestris. Plant Cell Tissue Organ Cult. 14:169–175; 1988.

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Nour, K.A., Thorpe, T.A. In vitro shoot multiplication of eastern white cedar (Thuja occidentalis). In Vitro Cell Dev Biol - Plant 29, 65–71 (1993). https://doi.org/10.1007/BF02632254

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Key words

  • eastern white cedar
  • Thuja occidentalis
  • axillary bud breaking
  • axillary shoots
  • clonal propagation