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Photoautotrophic micropropagation

  • Toyoki Kozai
Invited Review

Summary

Sugar in the medium is considered to be an essential cause for the high production costs of plantlets in conventional, heterotrophic micropropagation. Chlorophyllous explants, shoots, and plantlets in vitro have high photosynthetic ability to develop photoautotrophy, but their photosynthetic activity is restricted largely by the low CO2 concentration in the vessel during the photoperiod and in part by the presence of sugar in the medium. The growth of plantlets in vitro is often greater under photoautotrophic conditions than under heterotrophic conditions, provided that the in vitro environment is properly controlled for promoting photosynthesis. The advantages and disadvantages of photoautotrophic micropropagation are discussed.

Key words

photoautotrophic micropropagation CO2 enrichment plant tissue culture in vitro environment 

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Copyright information

© Tissue Culture Association 1991

Authors and Affiliations

  • Toyoki Kozai
    • 1
  1. 1.Department of Horticulture, Faculty of HorticultureChiba UniversityMatsudo, ChibaJapan

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