Human chorionic gonadotropin inhibits proliferation and induces expression of inhibin in human breast epithelial cells in vitro

  • M. V. Alvarado
  • N. E. Alvarado
  • J. Russo
  • I. H. Russo
Letter To The Editor


Mammary Gland Inhibin Human Breast Epithelial Cell Breast Epithelial Cell Line Bladder Carcinoma Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Alvarado, N. E.; Alvarado, M. V.; Russo, I. H., et al. Human chorionic gonadotropin (hCG) inhibits proliferation and induces the expression of inhibin in human breast epithelial cells (HBEC) in vitro. Proc. Am. Assoc. Cancer Res. 33:280; 1992 (Abstract).Google Scholar
  2. 2.
    Alvarado, M. V.; Russo, J.; Russo, I. H. Immunolocalization of inhibin in the mammary gland of rats treated with hCG. J. Histochem. Cytochem. 41:29–34; 1993.PubMedGoogle Scholar
  3. 3.
    Bicsak, T. A.; Tucker, E. M.; Cappel, S., et al. Hormonal regulation of granulosa cell inhibin biosynthesis. Endocrinology 119:2711–2719; 1986.PubMedGoogle Scholar
  4. 4.
    Czerwiec, F. S.; Meimer, M. H.; Puitt, D. Transiently elevated levels of c-fox and c-myc oncogene messenger ribonucleic acids in cultured murine Leydig tumor cells after addition of human chorionic gonadotropin. Mol. Endocrinol. 3:105–109; 1989.PubMedGoogle Scholar
  5. 5.
    Davis, S. R.; Burger, H. G.; Robertson, D. M., et al. Pregnant mare’s serum gonadotropin stimulates inhibin subunit gene expression in the immature rat ovary: dose response characteristics and relationships to serum gonadotropins, inhibin, and ovarian steroid content. Endocrinology 123:2399–2407; 1988.PubMedGoogle Scholar
  6. 6.
    Davis, S. R.; Dench, F.; Nikolaidis, I., et al. Inhibin ⋏-subunit gene expression in the ovaries of immature female rats is stimulated by pregnant mare serum gonadotropin. Biochem. Biophys. Res. Commun. 138:1191–1195; 1986.PubMedCrossRefGoogle Scholar
  7. 7.
    deJong, F. H. Inhibin. Physiol. Rev. 68:555–607; 1988.Google Scholar
  8. 8.
    Frazier, A. L.; Robbins, L. S.; Stork, P. J., et al. Isolation of TSH and LH/CG receptor cDNAs from human thyroid: regulation by tissue specific splicing. Mol. Endocrinol. 4:1264–1276; 1990.PubMedCrossRefGoogle Scholar
  9. 9.
    Ho, T. Y.; Alvarado, M. V.; Russo, J., et al. Human chorionic gonadotropin (hCG) stimulates inhibin synthesis in the rat ovary and mammary gland. Proc. Am. Asso. Cancer Res. 34:244; 1993 (Abstract).Google Scholar
  10. 10.
    Iles, R. K.; Purkis, P. E.; Whitehead, D. C., et al. Expression of beta human chorionic gonadotropin by non-trophoblastic non-endocrine “normal” and malignant epithelial cells. Br. J. Cancer 61:663–666; 1990.PubMedGoogle Scholar
  11. 11.
    Kurisaka, M.; Saitoh, S.; Seike, M., et al. Gonadotropin, as a tumor marker, in body fluid and tumor tissues of germ cell tumors. Brain and Nerve. 41:353–359; 1989.PubMedGoogle Scholar
  12. 12.
    Li, C. H.; Ramasharma, K. Inhibin. Ann. Rev. Pharmacol. Toxicol. 27:1–21; 1987.CrossRefGoogle Scholar
  13. 13.
    Mather, J. P.; Woodruff, T. K.; Krummen, L. A. Paracrine regulation of reproductive function by inhibin and activin. Minireview. Proc. Soc. Exp. Biol. Med. 201:1–15; 1992.Google Scholar
  14. 14.
    Matzuk, M. M.; Finegold, M. J.; Su, J-G. J., et al. α-Inhibin is a tumour suppressor gene with gonadal specificity in mice. Nature 360:313–319; 1992.PubMedCrossRefGoogle Scholar
  15. 15.
    Meunier, H.; Rivier, C.; Evans, R. M., et al. Gonadal and extragonadal expression of inhibin α, βA and βB subunits in various tissues predicts diverse functions. Proc. Natl. Acad. Sci. USA 85:247–251; 1988.PubMedCrossRefGoogle Scholar
  16. 16.
    Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Meth. 65:55–63; 1983.CrossRefGoogle Scholar
  17. 17.
    Murray-McIntosh, R. P.; Vos, M.; Lacy, M. P. Bovine inhibin immediately inhibits the electrophysiological response to chorionic gonadotropin in ovarian follicles of Xaenopus laevis. Endocrinology 128:3310–3312; 1991.PubMedGoogle Scholar
  18. 18.
    Petraglia, F.; Sawchenko, P.; Lim, A. T. W., et al. Localization, secretion and action of inhibin in human placenta. Science 237:187–192; 1987.PubMedCrossRefGoogle Scholar
  19. 19.
    Petraglia, F.; Vaughan, J.; Vale, W. Inhibin and activin modulate the release of gonadotropin-releasing hormone, human chorionic gonadotropin, and progesterone from cultured human placental cells. Proc. Natl. Acad. Sci. USA 86:5114–5117; 1989.PubMedCrossRefGoogle Scholar
  20. 20.
    Roberts, V.; Meunier, H.; Sawchenko, P. E., et al. Differential production and regulation of inhibin subunits in rat testicular cell types. Endocrinology 125:2350–2359; 1989.PubMedGoogle Scholar
  21. 21.
    Roberts, V. J.; Sawchenko, P. E.; Vale, W. Expression of inhibin/activin subunit messenger ribonucleic acids during rat embryogenesis. Endocrinology 128:3122–3129; 1991.PubMedGoogle Scholar
  22. 22.
    Russo, I. H.; Koszalka, M.; Gimotty, P. A., et al. Protective effect of chorionic gonadotropin on DMBA-induced mammary carcinogenesis. Br. J. Cancer 62:243–247; 1990.PubMedGoogle Scholar
  23. 23.
    Russo, I. H.; Koszalka, M.; Russo, J. Effect of human chorionic gonadotropin on mammary gland differentiation and carcinogenesis. Carcinogenesis 11:1849–1855; 1990b.PubMedCrossRefGoogle Scholar
  24. 24.
    Russo, I. H.; Koszalka, M.; Russo, J. Comparative study of the influence of pregnancy and hormonal treatment on mammary carcinogenesis. Br. J. Cancer 64:481–484; 1991.PubMedGoogle Scholar
  25. 25.
    Russo, J.; Russo, I. H. Role of differentiation on transformation on human breast epithelial cells. In: Medina, D.; Kidwell, W.; Heppner, G., et al., eds. Cellular and molecular biology of mammary cancer. New York: Plenum Publishing Co.; 1987:399–417.Google Scholar
  26. 26.
    Russo, J.; Russo, I. H. Boundaries in mammary carcinogenesis. In: Sudilovsky, O., ed. Boundaries between promotion and progression during carcinogenesis. New York: Plenum Press; 1991:43–59.Google Scholar
  27. 27.
    Soule, H. D.; Maloney, T. M.; Wolman, S. R., et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50:6075–6086; 1990.PubMedGoogle Scholar
  28. 28.
    Soule, H. D.; Vazquez, J.; Long, A., et al. A human cell line from a pleural effusion derived from a breast carcinoma. J. Natl. Cancer Inst. 51:1409–1416; 1973.PubMedGoogle Scholar
  29. 29.
    Woodruff, T. K.; Mayo, K. Regulation of inhibin synthesis in the rat ovary. Annu. Rev. Physiol. 52:807–821; 1990.PubMedCrossRefGoogle Scholar
  30. 30.
    Ziecick, A. J.; Stanchev, P. D.; Tilton, J. E. Evidence for the presence of luteinizing hormone/human chorionic gonadotropin-binding sites in the porcine uterus. Endocrinology 119:1159–1163; 1986.CrossRefGoogle Scholar

Copyright information

© Tissue Culture Association 1994

Authors and Affiliations

  • M. V. Alvarado
    • 1
  • N. E. Alvarado
    • 1
  • J. Russo
    • 1
  • I. H. Russo
    • 1
  1. 1.Department of PathologyFox Chase Cancer CenterPhiladelphia

Personalised recommendations