In Vitro Cellular & Developmental Biology

, Volume 24, Issue 5, pp 487–490 | Cite as

Cytoplasmic mediation of malignancy

  • Barbara A. Israel
  • Warren I. Schaeffer
Rapid Communications in Cell Biology
Accepted:

Summary

The relative roles of nucleus and cytoplasm in the induction and maintenance of the malignant state were studied. Cytoplasmic hybrid (cybrid) clones, derived from the fusion of cytoplasts from malignantly transformed cells to normal whole cells, produced tumors in 17% of the animals injected with them. Nuclear/cytoplasmic hybrid (reconstituted cell) clones, derived by fusion of cytoplasts from malignant cells with karyoplasts of normal cells, produced tumors in 97% of the animals injected. A unique aspect of this study is the fact that all of the cells utilized, both normal and malignantly transformed, were derived from an original cloned cells

Keywords

Parental Cell Modal Number Population Doubling Malignant Phenotype Early Passage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ozer, H. L. and K. K. Jha (1977) Adv. in Can. Res. 25:53.CrossRefGoogle Scholar
  2. 2.
    Groce, C. M., Hueber, K., Girardi, A. J., and H. Koprowski (1974) Cold Spring Harbor Symp. Quant. Biol. 39:335.Google Scholar
  3. 3.
    Borski, G., Blanchard, M. G., Youn, J. K., and B. Leon (1973) J. Natl. Cancer Inst., 51:781.Google Scholar
  4. 4.
    Howell, A. N., and R. Sager (1978) Proc Natl. Acad. Sci. USA 75:2238.CrossRefGoogle Scholar
  5. 5.
    Harris, H., Miller, O. J., Klein, G., Worst, P., and T. Tachibana (1969) Nature 223:363–368.PubMedCrossRefGoogle Scholar
  6. 6.
    Ziegler, M. L. (1978) Som. Cell Genet. 4:447–489.Google Scholar
  7. 7.
    Miller, R. A. and F. Ruddle (1976) Cell 9:44–55.CrossRefGoogle Scholar
  8. 8.
    Howell, N., and R. Sager (1979) Som. Cell Gen. 5:129–143.CrossRefGoogle Scholar
  9. 9.
    Klinger, H. P. (1980) Cytogenet. Cell Genet. 27:254–266.PubMedGoogle Scholar
  10. 10.
    Howell, A. N. and R. Sager (1978) Proc. Nat'l. Acad. Sci. 75:2358–2362.CrossRefGoogle Scholar
  11. 11.
    Koura, M., Isaka, H., Yoshida, M., Tosu, M. and T. Sekiguchi (1982) Gann 73:574–580.PubMedGoogle Scholar
  12. 12.
    Shay, J. W., Peters, T. T., Fuseler, J. W. (1978) Cell 14:835.PubMedCrossRefGoogle Scholar
  13. 13.
    Shay, J. W., and M. A. Clark (1979) J. Supramolecular Structure 11:33–49.CrossRefGoogle Scholar
  14. 14.
    Halaban, R., Moellmann, G., Godawska, E. and J. Eisenstadt (1980) Exp. Cell Res. 130:427.PubMedCrossRefGoogle Scholar
  15. 15.
    Weiss, M. C. and B. Ephrussi, (1966) Genetics, 54:1095.PubMedGoogle Scholar
  16. 16.
    Weiss, Mary C. and Howard Green, (1967) PNAS (USA), 58(3):1104–1111.CrossRefGoogle Scholar
  17. 17.
    Hayashi, J.-I., Gotoh, O., Tagashira, U., Tosu, M., and T. Sekiguchi (1980) FEBS Letters 117:59–62.PubMedCrossRefGoogle Scholar
  18. 18.
    Hayashi, J.-I., Tagashiro, Y., Higashida, H., Hirai, S.-I., Yoshida, M. and T. Sekiguchi (1984) Exp. Cell Res. 154:357–366.PubMedCrossRefGoogle Scholar
  19. 19.
    Ziegler, M. L. and R. L. Davidson, (1981) Som. Cell Genet., 7(1):73–88.CrossRefGoogle Scholar
  20. 20.
    Solus, J. F. and J. M. Eisenstadt (1984) Exp. Cell Res. 151:299–305.PubMedCrossRefGoogle Scholar
  21. 21.
    Perry, R. P., Kelley, D. E., Schibler, U., Huebner, K., and C. M. Groce (1979). J. Cell Physiol. 98:553–560.PubMedCrossRefGoogle Scholar
  22. 22.
    Ajiro, K., Zweidler, A., Borum, T., and C. M. Groce (1978) Proc. Natl. Acad. Sci. USA 75:5599–5603.PubMedCrossRefGoogle Scholar
  23. 23.
    Gaunt, S. J. and J. H. Subak-Sharpe (1979) Exp. Cell Res. 120:307–320.PubMedCrossRefGoogle Scholar
  24. 24.
    Bausher, J. and W. I. Schaeffer (1974) In Vitro 9:286–293.CrossRefGoogle Scholar
  25. 25.
    Schaeffer, W. I. (1980) In: Ca. Borek and G. M. Williams (eds.) Ann. N. Y. Acad. Sci. 349:165–182.Google Scholar
  26. 26.
    Israel, B. A. and W. I. Schaeffer (1987) In Vitro Cellular and Developmental Biology, 23(9):627–632.PubMedCrossRefGoogle Scholar
  27. 27.
    Clark, M. A., Crenshaw, A. H., and J. W. Shay (1978) Tissue Culture Assoc. Man. 4:801–804.CrossRefGoogle Scholar
  28. 28.
    Vindelov, L., Christensen, I. and N. Nissen (1983) Cytometry 3:328–331.PubMedCrossRefGoogle Scholar
  29. 29.
    DeMars, R. and K. Held (1972) 16:87–110.Google Scholar
  30. 30.
    Harris, M. (1975). J. Cell Physiol. 86:413–429.PubMedCrossRefGoogle Scholar
  31. 31.
    Wollgiehn, R. (1982) in L. Nover, M. Luckner, B. Parthier (eds.) Cell Differentiation: Molecular Basis and Problems, Springer-Verlag, Berlin, Heidelberg, pgs. 529–548.Google Scholar
  32. 32.
    McKinnell, R., B. A. Diggins and D. D. Labat (1969) Science 165:394–395.PubMedCrossRefGoogle Scholar
  33. 33.
    Mintz, B. (1977) in L. Nover and K. Mothis (eds.) Cell Differentiation in Microorganisms, Plants and Animals G. Fischer Verlag, Jena, pgs. 351–360.Google Scholar
  34. 34.
    Maan, S. S. (1979) Wheat Inf. Ser. 50:71–79.Google Scholar
  35. 35.
    Kihara, H. and K. Tsunewaki (1962) Jap. J. Genet. 37:310–313.Google Scholar
  36. 36.
    Matzinger, D. F. and L. G. Burk (1984) J. Hered. 75:167–170.Google Scholar
  37. 37.
    Mathias, R. J., K. Fukui and C. N. Law (1986) Theor. Appl. Genet. 72:70–75.CrossRefGoogle Scholar
  38. 38.
    Fischer Lindahl, K. and K. Burki, (1982) Proc. Nat'l. Acad. Sci. USA, 79:5362–5366.CrossRefGoogle Scholar
  39. 39.
    Fischer Lindahl, K. and B. Hausmann, (1983) Genetics, 103:483–494.Google Scholar
  40. 40.
    Abken, H., H. Jungfer, W. H. W. Albert and K. Willecke, (1986) J. Cell Biol., 103:795–805.PubMedCrossRefGoogle Scholar

Copyright information

© Tissue Culture Association, Inc. 1988

Authors and Affiliations

  • Barbara A. Israel
    • 1
  • Warren I. Schaeffer
    • 1
  1. 1.Department of MicrobiologyUniversity of Vermont, College of MedicineBurlington

Personalised recommendations