Cardiovascular Drugs and Therapy

, Volume 10, Issue 3, pp 331–339 | Cite as

EUK-8 a synthetic catalytic scavenger of reactive oxygen species protects isolated iron-overloaded rat heart from functional and structural damage induced by ischemia/reperfusion

  • S. Pucheu
  • F. Boucher
  • T. Sulpice
  • N. Tresallet
  • Y. Bonhomme
  • B. Malfroy
  • J. de Leiris
Ischemia and Reperfusion


The effects of EUK-8, a synthetic, catalytic scavenger of reactive oxygen species, on isolated iron-overloaded rat hearts submitted to ischemia-reperfusion were studied. In the absence of EUK-8, functional parameters (systolic and diastolic pressures, oxygen consumption as estimated by the product heart rate times left ventricular diastolic pressure) were severely impaired 1 minute and 15 minutes after reperfusion following a 15 minute ischemic episode. Dimethylthiourea (10 mM), a hydroxyl radical scavenger, had a minimally protective effect. In contrast, EUK-8 at a concentration of 50 μM in the perfusion medium maintained these parameters at close to their preischemia values. Electron microscopic analysis of heart tissues after 15 minutes ischemia followed by 15 minutes reperfusion showed extensive damage to mitochondria and sarcomeres in untreated hearts, while the extent of damage was significantly lower in EUK-8-treated hearts. The functional and structural protection afforded by EUK-8 were significantly better than those induced by dimethylthiourea. These data suggest that EUK-8 may be therapeutically useful in preventing heart damage induced by ischemia-reperfusion, for example, during thrombolytic treatment of myocardial infarction.

Key Words

ischemia-reperfusion oxygen free radicals isolated rat heart iron overload EUK-8 


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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • S. Pucheu
    • 1
  • F. Boucher
    • 1
  • T. Sulpice
    • 1
  • N. Tresallet
    • 1
  • Y. Bonhomme
    • 2
  • B. Malfroy
    • 3
  • J. de Leiris
    • 1
  1. 1.Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287Université Joseph FourierGrenobleFrance
  2. 2.Lipha, s.a.LyonFrance
  3. 3.EukarionBedfordUSA

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