In Vitro

, Volume 6, Issue 4, pp 257–265 | Cite as

Procedures to reduce contamination of cell cultures

  • Gerard J. McGarrity
  • Lewis L. Coriell
Article

Summary

The most frequent causes of cell culture contamination are poor techniques and housekeeping and inadequate sterility testing of supplies and culture media. The most common contaminants of cell cultures areMycoplasma, Torula sp., andPseudomonas sp. Routine procedures used in this laboratory to control or prevent accidental contamination include the use of filtered laminar air flow in transfer rooms, elimination of antibiotics wherever possible, careful execution of aseptic procedures, periodic review and discussion of techniques, sterility testing of all media components, use of approved clothing, and effective cleaning and disinfection procedures.

Keywords

Aseptic Technique Laminar Flow Hood Airborne Bacterium Airborne Route Tissue Culture Work 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Tissue Culture Association 1971

Authors and Affiliations

  • Gerard J. McGarrity
    • 1
  • Lewis L. Coriell
    • 1
  1. 1.Institute for Medical ResearchCamden

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