Rapid improvement in insulin binding to erythrocyte insulin receptors in non-insulin-dependent diabetes mellitus during therapy
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Insulin binding to erythrocyte receptors was studied in 36 newly diagnosed male subjects with NIDDM, treated with diet alone (Group I; n=10) or diet + glibenclamide (Group II; n=12) or diet + glibenclamide + metformin (Group III; n=14). Fourteen matched non-diabetic subjects were also studied as controls. Initially, mean (± SD) specific insulin binding was lower in NIDDM patients than in controls (p<0.001), due to decreased receptor number and affinity. Control of diabetes with short-term therapy (10 ± 2 days) resulted in significantly increased specific insulin binding in Groups II and III (p<0.001). A marginal increase was observed in Group I (p<0.01). The improved insulin binding observed in Group II and III patients after short-term therapy was maintained even after long-term therapy (9 ± 1 months). Analysis of the insulin binding data by Scatchard plots and average affinity profiles indicated increased receptor number and affinity after short-term therapy. However, changes in affinity were reversed with long-term therapy in Groups II and III and the predominant effect appeared to be an increase in the number of binding sites.
Key-wordsErythrocyte insulin receptors High-carbohydrate high-fiver diet (HCHF-diet) Insulin binding Long-term therapy Non-insulin-dependent diabetes mellitus (NIDDM) Oral drugs Rapid improvement Short-term therapy
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- 1.Beck-Nielsen H., Hjøllund E., Pedersen O., Richelsen B., Sørensen N. S.: Sulfonylureas improve insulin binding and insulin action in non-insulin-dependent diabetes mellitus — Diabet Care7 (Suppl.1), 100, 1984.Google Scholar
- 2.Beck-Nielsen H., Pedersen O., Lindskov H. O.: Increased insulin sensitivity and cellular insulin binding in obese diabetics following treatment with glibenclamide — Acta endocrinol. (Kbh.)90, 451, 1979.Google Scholar
- 7.Goldfine I. D., Iwamoto Y., Pezzino V., Trischitta V., Purrello F., Vigneri R.: Effects of biguanides and sulfonylureas on insulin receptors in cultured cells — Diabet Care7 (Suppl.1), 54, 1984.Google Scholar
- 9.Holle A., Dreyer M., Kuhnau J., Mangels W., Maack P., Siemers U., Rudger H. W.: Metformin increases the number of insulin receptors in human erythrocyte. In:Andreani D., De Pirro R., Lauro R., Olefsky J. M., Roth J. (Eds.): Current views on insulin receptors. Academic Press, London, 1981; p. 601.Google Scholar
- 18.Reaven G. M.: Insulin secretion and insulin action in non-insulin-dependent diabetes mellitus: which defect is primary? — Diabet Care7 (Suppl. 1), 17, 1984.Google Scholar
- 20.Turco G., Pegararo L., Giovinazzo B., D’Alberto M., Brossa C., Bruni B., Gamba S., Grassi G., Barbero P. L.: Decreased insulin binding to erythrocytes in diabetics with relative insulin resistance. In:Andreani D., De Pirro R., Lauro R., Olefsky J. M., Roth J. (Eds.): Current views on insulin receptors. Academic Press, London, 1981; p. 523.Google Scholar
- 21.Vigneri R., Pezzino V., Wong K. Y., Goldfine I. D.: Comparison of thein vitro effect of biguanides and sulphonylureas on insulin binding to its receptors in target cells — J. clin. Endocrinol.54, 95, 1982.Google Scholar
- 23.W.H.O. Expert Committee on Diabetes Mellitus.: W.H.O. Technical Report Series 727. Geneva, 1985.Google Scholar