Advertisement

Acta diabetologia latina

, Volume 25, Issue 3, pp 205–214 | Cite as

Rapid improvement in insulin binding to erythrocyte insulin receptors in non-insulin-dependent diabetes mellitus during therapy

  • Ambady Ramachandran
  • Lakshminarayanan Susheela
  • Viswanathan Mohan
  • Durgam A. S. Kuzhali
  • Moopil Viswanathan
Original Contributions
  • 23 Downloads

Summary

Insulin binding to erythrocyte receptors was studied in 36 newly diagnosed male subjects with NIDDM, treated with diet alone (Group I; n=10) or diet + glibenclamide (Group II; n=12) or diet + glibenclamide + metformin (Group III; n=14). Fourteen matched non-diabetic subjects were also studied as controls. Initially, mean (± SD) specific insulin binding was lower in NIDDM patients than in controls (p<0.001), due to decreased receptor number and affinity. Control of diabetes with short-term therapy (10 ± 2 days) resulted in significantly increased specific insulin binding in Groups II and III (p<0.001). A marginal increase was observed in Group I (p<0.01). The improved insulin binding observed in Group II and III patients after short-term therapy was maintained even after long-term therapy (9 ± 1 months). Analysis of the insulin binding data by Scatchard plots and average affinity profiles indicated increased receptor number and affinity after short-term therapy. However, changes in affinity were reversed with long-term therapy in Groups II and III and the predominant effect appeared to be an increase in the number of binding sites.

Key-words

Erythrocyte insulin receptors High-carbohydrate high-fiver diet (HCHF-diet) Insulin binding Long-term therapy Non-insulin-dependent diabetes mellitus (NIDDM) Oral drugs Rapid improvement Short-term therapy 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Beck-Nielsen H., Hjøllund E., Pedersen O., Richelsen B., Sørensen N. S.: Sulfonylureas improve insulin binding and insulin action in non-insulin-dependent diabetes mellitus — Diabet Care7 (Suppl.1), 100, 1984.Google Scholar
  2. 2.
    Beck-Nielsen H., Pedersen O., Lindskov H. O.: Increased insulin sensitivity and cellular insulin binding in obese diabetics following treatment with glibenclamide — Acta endocrinol. (Kbh.)90, 451, 1979.Google Scholar
  3. 3.
    Beck-Nielsen H., Pedersen O., Sørensen N. S.: Effects of dietary changes on cellular insulin binding andin vivo insulin sensitivity — Metabolism29, 482, 1980.PubMedCrossRefGoogle Scholar
  4. 4.
    De Fronzo R. A., Simonson D., Ferrannini E.: Hepatic and peripheral insulin resistance: a common feature of type 2 (non-insulin-dependent) and type 1 (insulin-dependent) diabetes mellitus — Diabetologia23, 313, 1982.CrossRefGoogle Scholar
  5. 5.
    De Meyts P., Roth J.: Co-operativity in ligand binding: a new graphic analysis — Biochem. biophys. Res. Commun.66, 1118, 1975.PubMedCrossRefGoogle Scholar
  6. 6.
    Gambhir K. K., Archer J. A., Carter L.: Insulin radioreceptor assay for human erythrocytes — Clin. Chem.23, 1590, 1977.PubMedGoogle Scholar
  7. 7.
    Goldfine I. D., Iwamoto Y., Pezzino V., Trischitta V., Purrello F., Vigneri R.: Effects of biguanides and sulfonylureas on insulin receptors in cultured cells — Diabet Care7 (Suppl.1), 54, 1984.Google Scholar
  8. 8.
    Greenfield M. S., Doberne L., Rosenthal M., Schulz B., Widstrom A., Reaven G. M.: Effects of sulfonylurea treatment onin vivo insulin secretion and action in patients with non-insulin-dependent diabetes mellitus — Diabetes31, 307, 1982.PubMedCrossRefGoogle Scholar
  9. 9.
    Holle A., Dreyer M., Kuhnau J., Mangels W., Maack P., Siemers U., Rudger H. W.: Metformin increases the number of insulin receptors in human erythrocyte. In:Andreani D., De Pirro R., Lauro R., Olefsky J. M., Roth J. (Eds.): Current views on insulin receptors. Academic Press, London, 1981; p. 601.Google Scholar
  10. 10.
    Hyvarinen A., Nikkila E. A.: Specific determination of blood glucose with ortho-toluidine — Clin. chim. Acta7, 140, 1962.PubMedCrossRefGoogle Scholar
  11. 11.
    Kolterman O. G., Gray R. S., Griffin J., Burstein P., Insel J., Scarlett J. A., Olefsky J. M.: Receptor and post-receptor defects contribute to the insulin resistance in non-insulin-dependent diabetes mellitus — J. clin. Invest.68, 957, 1981.PubMedCrossRefGoogle Scholar
  12. 12.
    Kolterman O. G., Greenfield M., Reaven G. M., Seakow M., Olefsky J. M.: Effect of a high carbohydrate diet on insulin binding to adipocytes and on insulin actionin vivo in man — Diabetes28, 731, 1979.PubMedCrossRefGoogle Scholar
  13. 13.
    Lebovttz H. E., Feinglos M. N.: Sulphonylurea drugs: mechanism of antidiabetic action and therapeutic usefulness — Diabet Care1, 89, 1978.CrossRefGoogle Scholar
  14. 14.
    Olczak S. A., Greenwood R. H., Hales C. N.: The effect of glibenclamide administration on insulin sensitivity and insulin binding to monocytes in normal subjects — Diabet Med.2, 352, 1985.PubMedCrossRefGoogle Scholar
  15. 15.
    Olefsky J. M., Reaven G. M.: Effects of sulphonylurea therapy on insulin binding to mononuclear leukocytes of diabetic patients — Amer. J. Med.60, 89, 1976.PubMedCrossRefGoogle Scholar
  16. 16.
    Prager R., Schernthaner G.: Comparison of insulin receptor binding to monocytes and erythrocytes in newly diagnosed type II diabetes mellitus — Hormone metabol. Res.14, 561, 1982.CrossRefGoogle Scholar
  17. 17.
    Reaven G. M.: Therapeutic approaches to reducing insulin resistance in patients with non-insulin-dependent diabetes mellitus — Amer. J. Med.74, 109, 1983.PubMedCrossRefGoogle Scholar
  18. 18.
    Reaven G. M.: Insulin secretion and insulin action in non-insulin-dependent diabetes mellitus: which defect is primary? — Diabet Care7 (Suppl. 1), 17, 1984.Google Scholar
  19. 19.
    Susheela L., Ramachandran A., Mohan V., Viswanathan M.: A semimicro-colorimetric method for estimating glycosylated haemoglobin — Indian J. med. Res.84, 93, 1986.PubMedGoogle Scholar
  20. 20.
    Turco G., Pegararo L., Giovinazzo B., D’Alberto M., Brossa C., Bruni B., Gamba S., Grassi G., Barbero P. L.: Decreased insulin binding to erythrocytes in diabetics with relative insulin resistance. In:Andreani D., De Pirro R., Lauro R., Olefsky J. M., Roth J. (Eds.): Current views on insulin receptors. Academic Press, London, 1981; p. 523.Google Scholar
  21. 21.
    Vigneri R., Pezzino V., Wong K. Y., Goldfine I. D.: Comparison of thein vitro effect of biguanides and sulphonylureas on insulin binding to its receptors in target cells — J. clin. Endocrinol.54, 95, 1982.Google Scholar
  22. 22.
    Ward C. M., Harrison L. C.: Structure of the human erythrocyte insulin receptor — Diabetes35, 101, 1986.PubMedCrossRefGoogle Scholar
  23. 23.
    W.H.O. Expert Committee on Diabetes Mellitus.: W.H.O. Technical Report Series 727. Geneva, 1985.Google Scholar

Copyright information

© Casa Editrice «il Ponte» 1988

Authors and Affiliations

  • Ambady Ramachandran
    • 1
  • Lakshminarayanan Susheela
    • 1
  • Viswanathan Mohan
    • 1
  • Durgam A. S. Kuzhali
    • 1
  • Moopil Viswanathan
    • 1
  1. 1.Diabetes Research Centre and M. V. Hospital for DiabetesRoyapuram, MadrasIndia

Personalised recommendations