Acta diabetologia latina

, Volume 25, Issue 3, pp 205–214 | Cite as

Rapid improvement in insulin binding to erythrocyte insulin receptors in non-insulin-dependent diabetes mellitus during therapy

  • Ambady Ramachandran
  • Lakshminarayanan Susheela
  • Viswanathan Mohan
  • Durgam A. S. Kuzhali
  • Moopil Viswanathan
Original Contributions


Insulin binding to erythrocyte receptors was studied in 36 newly diagnosed male subjects with NIDDM, treated with diet alone (Group I; n=10) or diet + glibenclamide (Group II; n=12) or diet + glibenclamide + metformin (Group III; n=14). Fourteen matched non-diabetic subjects were also studied as controls. Initially, mean (± SD) specific insulin binding was lower in NIDDM patients than in controls (p<0.001), due to decreased receptor number and affinity. Control of diabetes with short-term therapy (10 ± 2 days) resulted in significantly increased specific insulin binding in Groups II and III (p<0.001). A marginal increase was observed in Group I (p<0.01). The improved insulin binding observed in Group II and III patients after short-term therapy was maintained even after long-term therapy (9 ± 1 months). Analysis of the insulin binding data by Scatchard plots and average affinity profiles indicated increased receptor number and affinity after short-term therapy. However, changes in affinity were reversed with long-term therapy in Groups II and III and the predominant effect appeared to be an increase in the number of binding sites.


Erythrocyte insulin receptors High-carbohydrate high-fiver diet (HCHF-diet) Insulin binding Long-term therapy Non-insulin-dependent diabetes mellitus (NIDDM) Oral drugs Rapid improvement Short-term therapy 


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Copyright information

© Casa Editrice «il Ponte» 1988

Authors and Affiliations

  • Ambady Ramachandran
    • 1
  • Lakshminarayanan Susheela
    • 1
  • Viswanathan Mohan
    • 1
  • Durgam A. S. Kuzhali
    • 1
  • Moopil Viswanathan
    • 1
  1. 1.Diabetes Research Centre and M. V. Hospital for DiabetesRoyapuram, MadrasIndia

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