Acta diabetologia latina

, Volume 21, Issue 1, pp 47–56 | Cite as

Effect of estrogens on blood sugar, serum insulin and serum free fatty acids, and pancreas cytology in female dogs

  • Aurora Renauld
  • Irene von Lawzewitsch
  • Ricardo L. Pérez
  • Rita Sverdlik
  • Alicia Agüero
  • Virgilio G. Foglia
  • Ricardo R. Rodríguez
Original Contributions


We analyzed the changes in blood sugar (BS), serum immunoreactive insulin (IRI), circulating free fatty acids (FFA) and pancreatic cytology caused by estrogenization at low pharmacological dosage in female dogs. Vehicle-injected and untreated controls (anestrus) were studied as well. Neither mean basal BS nor basal serum IRI was modified by the treatments, while the mean basal serum FFA value was raised. Glucose tolerance was not modified by the estrogens while glucosey-mean was significantly raised. Hyperglycemia was higher for a longer time in estrogenized animals compared to both controls, while the profiles of hyperinsulinemia coincided. In the estrogen-treated bitches, the pancreatic B-cells contained scarse brown-stained granules near their vascular pole, as shown by an immunochemical method. In the peripheral part of the pancreas, near the acini, some solitary, poorly β-granulated B-cells were present. During the IVGTT, serum FFA reached lower values for a longer time in the estrogenized bitches as compared to those found in both control groups. Insulin-induced hypoglycemia in the estrogenized animals coincided with the one evoked in the vehicle controls; in the semilog relationship of serum IRI and time,y-mean was lower than that observed in oil-injected controls, and insulin space was larger. The serum FFA levels of the estrogenized bitches, very high in the basal conditions, did not respond to insulin administration, and were above those found in untreated controls and also in vehicle-injected controls just at the beginning of the test. These results are discussed. We came to the conclusion that estrogenization causes some glucose intolerance in bitches while insulin sensitivity remains normal in the IVITT as studied measuring BS. The glucose intolerance is thought to be related to a reduction in glucose space and occurs despite the normality of the serum IRI response. The pancreas must have an intense secretory responsein vivo so as to maintain normal IRI activity despite degranulation of the islets of Langerhans and poor islet hypertrophy and neoformation. The serum FFA changes are thought to contribute towards the tendency to adiposity in these animals.


17β-estradiol Blood sugar Estrogens Intravenous glucose tolerance test (IVGTT) Intravenous insulin tolerance test (IVITT) Serum free fatty acids Serum immunoreactive insulin 


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  1. 1.
    Bailey C. J., Matty A. J.: The effect of sex hormones on plasma insulin and glucose to-lerance in the female rat - J. Endocr.51, xxi, 1971.PubMedGoogle Scholar
  2. 2.
    Bancroft H.: Introducción a la bioestadística. Ed. Universitaria, Buenos Aires, 1960.Google Scholar
  3. 3.
    Baum G. J., Meyer R. K.: Effect of adrenal steroids and diethylstilbestrol on growth and fat content of cockerels - Amer. J. Physiol.198, 1263, 1960.PubMedGoogle Scholar
  4. 4.
    Beck P., Wells S.: Plasma insulin responses to glucose in estradiol-and mestranol-treated Rhesus monkeys - Clin. Res.16, 338, 1968.Google Scholar
  5. 5.
    Bertoli A., De Pirro R., Greco A. V., Magnatta R., Lauro R.: Differences in insulin receptors between men and menstruating women and influence of sex hormones in insulin binding during the menstrual cycle - J. clin. Endocr.50, 246, 1980.PubMedGoogle Scholar
  6. 6.
    Bottiglioni F., Flamigni C.: Influence des différents traitements hormonaux sur l’ilot de Langerhans - Gynéc. et Obstét.62, 607, 1963.Google Scholar
  7. 7.
    Byrnes W. W., Meyer R. K.: The inhibition of gonadotrophic hormone secretion by physiological doses of estrogens - Endocrinology48, 133, 1951.PubMedGoogle Scholar
  8. 8.
    Campbell J., Rastogi K. S.: Effect of growth hormone on the rate of disappearance of insulin from blood in depancreatized and Houssay dogs - Metabolism16, 562, 1967.PubMedCrossRefGoogle Scholar
  9. 9.
    Christie D. W., Bell E. T., Horth C. E., Palmer R. F.: Peripheral plasma progesterone levels during the canine oestrous cycle - Acta endocr. (Kbh.)68, 543, 1971.Google Scholar
  10. 10.
    Conard V.: Mesure de l’assimilation du glucose. Bases théoriques et applications cliniques - Acta gastro-ent. belg.18, 803, 1955.Google Scholar
  11. 11.
    Costrini N. V., Kalkhoff R. K.: Relative effects of pregnancy, estradiol, and progesterone on plasma insulin and pancreatic islet insulin secretion - J. clin. Invest.50, 992, 1971.PubMedGoogle Scholar
  12. 12.
    De Bodo R. C., Altszuler N.: Insulin hypersensitivity and physiological insulin antagonists - Physiol. Rev.38, 389, 1958.Google Scholar
  13. 13.
    De Pirro R., Forte F., Bertoli A., Greco A. V., Lauro R.: Changes in insulin receptors during oral contraception - J. clin. Endocr.52, 29, 1981.PubMedGoogle Scholar
  14. 14.
    Dixon W. J., Massey F. J. Jr.: Introduction to statistical analysis. 2nd ed. McGraw-Hill Book Co., New York, 1957; p. 194.Google Scholar
  15. 15.
    Erlandsen S. L., Hecre O. D., Parson J. A., McEvoy R. C., Elde R. P.: Distribution of cell types in the islet and evidence for the presence of somatostatin and gastrin within the D cell - J. Histochem. Cytochem.24, 883, 1976.PubMedGoogle Scholar
  16. 16.
    Erlandsen S. L., Parson J. A., Burke J. P., Redick J. A., van Orden D. E., van Orden L. S.: A modification of the unlabeled antibody enzyme method using heterologous antisera for the light microscope and ultrastructural localization of insulin, glucagon and growth hormone - J. Histochem. Cytochem.23, 666, 1975.PubMedGoogle Scholar
  17. 17.
    Florentin P. D., Picard D.: Recherches histologiques sur les signes d’activité du pancréas endocrine - C.R. Soc. Biol. (Paris)121, 90, 1936.Google Scholar
  18. 18.
    Gabe M.: Techniques histologiques. Masson et Cie. Éd., Paris, 1968; p. 363.Google Scholar
  19. 19.
    Goetz F. C., Greenberg B. Z., Ells J., Meinert C.: A simple immunoassay for insulin: application to human and dog plasma - J. clin. Endocr.23, 1237, 1963.PubMedCrossRefGoogle Scholar
  20. 20.
    Graham B. C., Karnovsky M. J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubulus of mouse kidney: ultrastructural cytochemistry by a new technique - J. Histochem. Cytochem.14, 291, 1966.PubMedGoogle Scholar
  21. 21.
    Haist R. E.: Factors affecting the islets of Langerhans - Diabetes2, 295, 1953.PubMedGoogle Scholar
  22. 22.
    Houssay B. A., Foclia V. G., Rodriguez R. R.: Production or prevention of some types of experimental diabetes by estrogens and corticosteroids - Acta endocr. (Kbh.)17, 146, 1954.Google Scholar
  23. 23.
    Itaya K., Ut M.: Colorimetric determination of free fatty acids in biological fluids - J. Lipid Res.6, 16, 1965.PubMedGoogle Scholar
  24. 24.
    Kochakian C. D.: The protein anabolic effects of steroid hormones - Vitam. and Horm.4, 255, 1946.CrossRefGoogle Scholar
  25. 25.
    Krauth M. C., Schillinger E.: Changes in insulin receptor concentration in rat fat cells by the treatment with the gestagens clomegestone acetate and cyproterone acetate - Acta endocr. (Kbh.)86, 667, 1977.Google Scholar
  26. 26.
    Létard E., Szumowski P., Théret M.: Applications des oestrogènes de synthèse dans l’accéleration de la croissance et de l’engraissement du porc - Rev. Méd. vét.128, 65, 1952.Google Scholar
  27. 27.
    Lison L.: Statistique appliquée à la biologie expérimentale. Gauthier-Villars, Paris, 1958; p. 132.Google Scholar
  28. 28.
    Nakane P. K.: Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method - J. Histochem. Cytochem.16, 577, 1968.Google Scholar
  29. 29.
    Renauld A., Andrade L. L., Lindental D. M., Garcia Ben M. S.: Micrométodo automatizado para la medición de la glucemia. Estudio comparativo de conservadores - Rev. Asoc. bioquím. argent.40, 171, 1975.Google Scholar
  30. 30.
    Renauld A., Sverdlik R. C., Agüero A., Rodríguez R. R., Foglia V. G.: Serum insulin, free fatty acids and blood sugar during the estrous cycle of dogs - Hormone metab. Res. 1982 (In press).Google Scholar
  31. 31.
    Renauld A., Sverdlik R. C., Fels R. C., von Lawzewitsch I., Pérez R. L., Foglia V. G., Rodríguez R. R.: Metabolic and histological pancreatic changes induced by orchidectomy in dogs - Hormone metab. Res.12, 370, 1980.CrossRefGoogle Scholar
  32. 32.
    Renauld A., von Lawzewitsch I., Pérez R. L., Sverdlik R. C., Rodríguez R. R., Foglia V. G.: Algunos aspectos endocrino-metabólicos del tratamiento con estrógenos en perros machos - 14th Lat.-Amer. Congr. of Physiological Sciences, São Paulo 1981; p. 219.Google Scholar
  33. 33.
    Schutte A. P.: Canine vaginal cytology. I) Technique and cytological morphology; II) Cyclic changes; III) Compilation and evaluation of cellular indices - J. small Anim. Pract.8, 301, 1967.PubMedGoogle Scholar
  34. 34.
    Shamoon H., Felig P.: Effects of estrogens on glucose uptake by rat muscle - Yale J. Biol. Med.47, 227, 1974.PubMedGoogle Scholar
  35. 35.
    Talaat A. M., Habib Y. A., Malek A.: Effect of estradiol dipropionate on the carbohydrate metabolism in the liver and muscle of adult female rabbits - Arch. int. Pharmacodyn.123, 490, 1960.PubMedGoogle Scholar
  36. 36.
    Vazquez Lopez E.: Golgi apparatus as an indicator of secretory activity in pancreatic islet cells - Nature (Lond.)146, 589, 1940.Google Scholar
  37. 37.
    Weir E. E., Pretlov T. G., Pitts A., Williams E. E.: A more sensitive and specific immunocytochemical peroxidase stain for the localization of cellular antigen by the enzyme-antibody conjugated method - J. Histochem. Cytochem.22, 1135, 1974.PubMedGoogle Scholar

Copyright information

© Casa Editrice «il Ponte» 1983

Authors and Affiliations

  • Aurora Renauld
    • 1
    • 2
  • Irene von Lawzewitsch
    • 1
    • 2
  • Ricardo L. Pérez
    • 1
    • 2
  • Rita Sverdlik
    • 1
    • 2
  • Alicia Agüero
    • 1
    • 2
  • Virgilio G. Foglia
    • 1
    • 2
  • Ricardo R. Rodríguez
    • 1
    • 2
  1. 1.Instituto de Fisiología ‘Prof. Dr. Bernardo A. Houssay’ Facultad de MedicinaUniversidad de Buenos AiresBuenos Aires
  2. 2.Instituto de Histología y Embriología (E.H.I.G.E.) Instituto de Obstetricia y Patología de la Reproducción Facultad de Ciencias VeterinariasUniversidad de Buenos AiresBuenos Aires

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