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Initiation and ultrastructure of a reptilian fibroblast cell line obtained from cutaneous fibropapillomas of the green turtle,Chelonia mydas

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Summary

Two fibroblastic cell lines were established from explants of fibropapillomas of each of two different green turtles (Chelonia mydas). These cells, designated GTFP (Green Turtle Fibropapilloma), were subcultured approximately 30 times at 30°C in Eagle’s minimal essential media supplemented with 2 to 10% fetal bovine serum. The ultrastructural morphology of the cultured fibroblasts is described. The cells contained abundant rough endoplasmic reticulum, polyribosomes, and mitochondria; collagen fibrils were visible in the extracellular space. No viruslike particles or evidence of other pathogenic agents could be demonstrated by electron microscopy in any of the cultured cells examined.

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Author information

Correspondence to Elliott R. Jacobson.

Additional information

Supported in part by a grant from Sea World Research Institute, Hubbs Marine Research Center, San Diego, California 92109, and the Chelonia Institute, Arlington, Virginia 22209. Published as University of Florida, College of Veterinary Medicine, Journal Series no. 192.

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Mansell, J.L., Jacobson, E.R. & Gaskin, J.M. Initiation and ultrastructure of a reptilian fibroblast cell line obtained from cutaneous fibropapillomas of the green turtle,Chelonia mydas . In Vitro Cell Dev Biol 25, 1062–1064 (1989). https://doi.org/10.1007/BF02624142

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Key words

  • fibropapilloma
  • cell line
  • ultrastructure
  • green turtle
  • Chelonia mydas