In Vitro Cellular & Developmental Biology

, Volume 25, Issue 11, pp 980–986 | Cite as

Locomotory behavior of fibroblasts in “sail-sheets”

  • Satish C. Tripathi
Regular Papers
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Accepted:

Summary

The locomotory behavior of fibroblasts in two-dimensional cultures (e.g., on culture dishes, cover slips, etc.) was first reported by Abercrombie’s group. This paper describes some of the features of movement of fibroblasts in a unique culture system known as sail-sheet cultures (SSCs). Cells in SSCs grow mostly on one another in a three-dimensional form that resembles, to some extent, the in vivo situation. We grew chicken heart fibroblasts (CHFs) as SSCs and studied their locomotory behavior by time-lapse filming extended for periods ranging from 12 h to several days. It was found that CHFs grown as sail-sheets exhibit many features of movement as observed in conventional two-dimensional cultures (CCs). However, we observed that CHFs in SSCs, like those in vivo, lack leading lamella directing their movement. Furthermore, locomotion is significantly slower in SSCs than in CCs. Based on data on the movement of CHFs within the mesh holes of inert grids, we suggest that the mesenchymal cells in SSCs, in addition to their individual movement, move in sheets and that their movement in sheets results in the closure of the mesh holes, a situation that resembles the phenomenon of healing of wounds. Thus SSCs provide a model system for the study of healing of wounds. The presence of collagenlike extracellular matrix (CLECM) between cellular layers in SSCs suggests that CLECM may be involved in guiding the locomotory behavior of CHFs in SSCs.

Key words

sail-sheet cultures conventional cultures chicken heart fibroblasts transmission electron microscopy collagenlike extracellular matrix 
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References

  1. 1.
    Abercrombie, M.; Heaysman, J. E. M.; Pegrum, S. M. The locomotion of fibroblasts in culture. I. Movement of the leading edge. Exp. Cell Res. 59:393–398; 1970.PubMedCrossRefGoogle Scholar
  2. 2.
    Harrison, R. G. The reaction of embryonic cells to solid structure. J. Exp. Zool. 17:521–544; 1914.CrossRefGoogle Scholar
  3. 3.
    Leighton, J. A sponge matrix method for tissue culture. Formation of organized aggregates of cellsin vitro. JNCI 12:545–561; 1951.PubMedGoogle Scholar
  4. 4.
    Curtis, A. S. G.; Varde, M. Control of cell behavior: topographical factors. JNCI 33:15–26; 1964.PubMedGoogle Scholar
  5. 5.
    Ehrman, R. L.; Gey, G. O. The growth of cells on a transparent gel of reconstituted rat tail collagen. JNCI 16:1375–1404; 1956.Google Scholar
  6. 6.
    Elsdale, T.; Bard, J. Collagen substrata for studies on cell behavior. J. Cell Biol. 54:626–637; 1972.PubMedCrossRefGoogle Scholar
  7. 7.
    Curtis, A. S. G.; Seehar, G. M. The control of cell division by tension or diffusion. Nature 274:52–53; 1978.PubMedCrossRefGoogle Scholar
  8. 8.
    Tripathi, S. C.; Curtis, A. S. G. Cell behavior: a new culture technique yielding new data. Proceedings of Michael Abercrombie Memorial International Meeting of British Society of Cell Biology held at University College, London. 1982:22.Google Scholar
  9. 9.
    Tripathi, S. C.; Kerr, J. Effect of mechanical stress on cellular morphology. Tissue & Cell. In press.Google Scholar
  10. 10.
    Tripathi, S. C. Investigations on the control of cell behavior and the cell cycle. Scotland: University of Glasgow; 1984. Thesis.Google Scholar
  11. 11.
    Dunn, G. A.; Heath, J. P. A new hypothesis of contact guidance in tissue cells. Exp. Cell Res. 101:1–14; 1976.PubMedCrossRefGoogle Scholar
  12. 12.
    Dunn, G. A.; Ebendal T. Contact guidance on oriented collagen gels. Exp. Cell Res. 111:475–479; 1978.PubMedCrossRefGoogle Scholar
  13. 13.
    Mautner, V.; Hynes, R. O. Surface distribution of LETS protein in relation to the cytoskeleton of normal and transformed cells. J. Cell Biol. 75:743–768; 1977.PubMedCrossRefGoogle Scholar
  14. 14.
    Bard, J. B.; Hay, E. D. The behavior of fibroblasts from the developing avian cornea. Morphology and movementin situ andin vitro. J. Cell Biol. 67:400–418; 1975.PubMedCrossRefGoogle Scholar
  15. 15.
    Abercrombie, M.; Gitlin, G. The locomotory behavior of small group of fibroblasts. Proc. R. Soc. Lond. Biol. 162:289–302; 1965.CrossRefGoogle Scholar
  16. 16.
    Vasiliev, J. M.; Gelfand, I. M.; Domnina, L. V., et al. Effect of colcemid on the locomotory behavior of fibroblasts. J. Embryol. Exp. Morphol. 24:625–640; 1970.PubMedGoogle Scholar
  17. 17.
    Osborn, M.; Weber, K. Cytoplasmic microtubules in tissue culture cells appear to grow from an organizing structure towards the plasma membrane. Proc. Natl. Acad. Sci. USA 73:867–871; 1976.PubMedCrossRefGoogle Scholar
  18. 18.
    Seehar, G. M. The control of cell cycle in fibroblasts. Scotland: University of Glasgow; 1978. Thesis.Google Scholar
  19. 19.
    Abercrombie, M.; Heaysman, J. E. M.; Karthauser, H. M. Social behavior of cells in tissue culture. III. Mutual influence of sarcoma cells and fibroblasts. Exp. Cell Res. 13:276–291; 1957.PubMedCrossRefGoogle Scholar
  20. 20.
    Vesely, P.; Weiss, R. A. Cell locomotion and contact inhibition of normal and neoplastic rat cells. Intl. J. Cancer 11:64–76; 1973.CrossRefGoogle Scholar
  21. 21.
    Abercrombie, M.; James, D. W.; Newcombe, J. F. Wound contraction in rabbit skin, studied by splinting the wound margins. J. Anat. 94:170–182; 1960.PubMedGoogle Scholar
  22. 22.
    Vakaet, L. Some new data concerning the formation of the definitive endoblast in the chick embryo. J. Embryol. Exp. Morphol. 10:38–57; 1962.PubMedGoogle Scholar

Copyright information

© Tissue Culture Association, Inc 1989

Authors and Affiliations

  • Satish C. Tripathi
    • 1
  1. 1.Center for Environmental Health SciencesMassachusetts Institute of TechnologyCambridge

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