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In Vitro Cellular & Developmental Biology

, Volume 26, Issue 12, pp 1186–1194 | Cite as

Routine culturing of normal, dysplastic and malignant human mammary epithelial cells from small tissue samples

  • Joanne T. Emerman
  • Darcy A. Wilkinson
Rapid Communications in Cell Biology

Summary

We compared the growth and morphology of normal, dysplastic and malignant human mammary epithelial cells (HMEC) in medium containing 5% human serum, a serum-free medium (32) and serum-free medium with a low Ca++ concentration. Tissues were dissociated and epithelial organoids or single cells were seeded onto collagen-coated dishes. The cells grew in serum-containing medium, but growth of fibroblasts was also stimulated. The serum-free medium consistently selected for and stimulated the growth of epithelial cells. There was little advantage in reducing the Ca++ concentration to further increase cell yield. This serum-free primary culture system allows us to routinely prouce sufficient numbers of HMEC from small tissue samples for molecular biological investigations. Furthermore, the maintenance of cells in a defined medium can provide a system for evaluating the direct effects of factors on gene expression.

Key words

human mammary epithelial cells primary culture serum-free medium 

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Copyright information

© Tissue Culture Association 1990

Authors and Affiliations

  • Joanne T. Emerman
    • 1
  • Darcy A. Wilkinson
    • 1
  1. 1.Department of AnatomyUniversity of British ColumbiaVancouverCanada

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