In Vitro Cellular & Developmental Biology

, Volume 24, Issue 7, pp 699–704 | Cite as

Control of angiotensinogen production by H4 rat hepatoma cells in serum-free culture

  • Masatoshi Togami
  • Daphne Blazka
  • Jun Hayashi
Regular Papers
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Summary

A serum-free, hormone and attachment factor supplemented culture for rat H4 hepatoma cells was established. In the defined medium (Dulbecco's Modified Eagle's +Ham's F12+insulin, transferrin, fibronectin liver cell growth factor, and sodium selenite), H4 cells grew equally well as in 10% fetal bovine serum supplemented medium. H4 cells in either defined or serum-containing culture conditions produce transferrin but not albumin or alpha-fetoprotein. In this paper we have studied the effect of various hormones and pressor peptides on the production of angiotensinogen by H4 cells cultured in defined conditions. Only glucocorticoid hormone had a significant effect on the production of angiotensinogen, whereas other hormones previously reported to exert their effect on angiotensinogen production had little or no effect.

Key words

H4 rat hepatoma angiotensinogen serum-free culture glucocorticoid 
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Copyright information

© Tissue Culture Association, Inc 1988

Authors and Affiliations

  • Masatoshi Togami
    • 1
  • Daphne Blazka
    • 2
  • Jun Hayashi
    • 2
  1. 1.Japan Synthetic Rubber Co., Ltd.KanawaJapan
  2. 2.W. Alton Jones Cell Science Center, Inc.Lake Placid

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