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In Vitro Cellular & Developmental Biology

, Volume 23, Issue 1, pp 67–70 | Cite as

Direct quantification of in vitro cell growth through image analysis

  • Mary Ann Lila Smith
  • L. Art Spomer
Rapid Communications in Cell Biology

Summary

Objective, accurate, non-intrusive measurement of in vitro cell growth was realized through microcomputerized video image analysis. Recently-released video and digitizing hardware and software were incorporated into an analytical system which accurately quantified visual differences between cultures on a cell number or fresh mass basis. Sequential measurements during culture incubation further detected and quantified subtle changes in colony area and density resulting from growth. Each measurement was acquired rapidly, without encroaching on the in vitro environment, so cell growth was undisturbed. Custom software routines coordinated the quantification of this detailed record into precise cumulative growth curves.

Key words

densitometry growth image analysis quantification video digitization 

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Copyright information

© Tissue Culture Association, Inc 1987

Authors and Affiliations

  • Mary Ann Lila Smith
    • 1
  • L. Art Spomer
    • 1
  1. 1.Department of HorticultureUniversity of IllinoisUrbana

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