In Vitro Cellular & Developmental Biology

, Volume 22, Issue 4, pp 180–192

Reaggregation of fetal rat brain cells in a stationary culture system I: Methodology and cell identification

  • Rolf Bjerkvig
  • Sverre K. Steinsvåg
  • Ole Didrik Laerum
Invited Review

DOI: 10.1007/BF02623302

Cite this article as:
Bjerkvig, R., Steinsvåg, S.K. & Laerum, O.D. In Vitro Cell Dev Biol (1986) 22: 180. doi:10.1007/BF02623302

Summary

A stationary tissue culture system for reaggregation cultures of rat brain cells is described. Aggregates were formed by placing cells at high concentrations in liquid overlay cultures on a nonadherent nutrient agar surface. No physical stress in the form of rotation or shaking was applied to the aggregating cell population.

Transmission electron microscopy and immunohistochemistry showed that the cells developed from homogeneously dispersed, immature cells in Day 4 aggregates, to mature astrocytes, oligodendrocytes, and neurons in Day 20 aggregates. Twenty days and older aggregates had a tightly packed neuropil which was most prominent in a cell-sparse outer layer of the aggregates. When the aggregates were allowed to adhere to a substrate, both glial fibrillary acidic protein (GFAP) positive and negative cells were observed migrating out from the aggregates. Cells giving a positive reaction for neuron specific enolase (NSE) were also present. This reaggregation procedure, with transfer of selected brain cell aggregates into agar-coated multiwells is an alternative three-dimensional culture system which can be potentially useful in the study of morphogenesis and cell interactions in the nervous system.

Key words

reaggregation fetal rat brain cells stationary tissue culture cell differentiation 

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Copyright information

© Tissue Culture Association, Inc 1986

Authors and Affiliations

  • Rolf Bjerkvig
    • 1
  • Sverre K. Steinsvåg
    • 1
  • Ole Didrik Laerum
    • 1
  1. 1.The Gade Institute, Department of pathologyUniversity of Bergen, N-5016 Haukeland HospitalBergenNorway

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