In Vitro Cellular & Developmental Biology

, Volume 23, Issue 8, pp 546–558

In vitro modulation of antioxidant enzymes in normal and malignant renal epithelium

  • An Hang Yang
  • Terry D. Oberley
  • Larry W. Oberley
  • Steven M. Schmid
  • Kenneth B. Cummings
Article

Summary

The activities of three antioxidant enzymes, superoxide dismutase, catalase, and glutathione peroxidase, were monitored in isolated human renal adenocarcinoma tissues and in cultured human renal adenocarcinoma cells. The results were compared to the activities of these enzymes in the proposed cell of origin, isolated human proximal tubular tissues, and cultured proximal tubular epithelial cells. Strong modulation of these enzymes by culture conditions was observed in normal cells but not in carcinoma cells. Low levels of cellular lipid peroxidation, as assessed by levels of malondialdehyde (MDA), were observed in adenocarcinoma cells under the culture conditions tested with one exception: greatly elevated MDA was observed in renal adenocarcinoma cells growth on plastic in serum-free, chemically defined medium. This increased lipid peroxidation correlated with a loss of cell viability under these conditions.

Key words

antioxidant enzymes renal epithelium 

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Copyright information

© Tissue Culture Association, Inc 1987

Authors and Affiliations

  • An Hang Yang
    • 1
  • Terry D. Oberley
    • 1
    • 2
  • Larry W. Oberley
    • 3
  • Steven M. Schmid
    • 4
  • Kenneth B. Cummings
    • 4
    • 5
  1. 1.Department of PathologyUniversity of Wisconsin Medical SchoolMadison
  2. 2.Laboratory Service, Electron Microscopy SectionWilliam S. Middleton Memorial Veterans HospitalMadison
  3. 3.Radiation Research LaboratoryUniversity of Iowa College of MedicineIowa City
  4. 4.Department of Human OncologyUniversity of Wisconsin Medical SchoolMadison
  5. 5.Department of SurgeryUniversity of Wisconsin Medical SchoolMadison

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