Tissue culture of human and canine thoracic duct endothelium
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Summary
Endothelial cells from the canine or human thoracic duct were harvested using 0.2% collagenase digestion and grown in Media 199, supplemented with fetal bovine serum. The canine endothelial cells grew to confluence (4.4 to 12×104 cells/cm2) in 6 to 10 d; doubling times ranged from 1.5 to 2.8 d. There was a minimum critical density for cell growth between 500 and 10 000 cells/cm2. The canine endothelial cells have been maintained in culture for periods up to 11 mo. The human thoracic duct endothelial cells are more difficult to grow and maintain. Endothelial cells were isolated from 5 out of 35 human thoracic ducts and grew for periods of up to 2 wk before degenerating. Both human and canine endothelial cells were Factor VIII positive. It has thus been demonstrated that it is possible to grow canine and, less easily, human thoracic duct endothelium in tissue culture.
Key words
lymphatic endothelium thoracic duct tissue culturePreview
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References
- 1.Bowman, C. A.; Witte, M. H.; Witte, C. L.; Way, D. L.; Nagle, R. B.; Copeland, J. G.; Daschbach, C. C. Cystic hygroma reconsidered: Hamartoma or neoplasm? Primary culture of an endothelial cell line from a massive cervicomediastinal hygroma with bony lymphangiomatosis. Lymphology 17:15–22; 1984.PubMedGoogle Scholar
- 2.Folkman, J.; Haudenschild, C. C.; Zetter, B. R. Long-term culture of capillary endothelial cells. Proc. Natl. Acad. Sci. USA 76:5217–5221; 1979.PubMedCrossRefGoogle Scholar
- 3.Freshney, R. I.; Frame, M. C. Culture of endothelial cells for the study of angiogenesis in vitro. In: Thilo-Korner, D. G. S.; Freshney R. I., eds. The endothelial cell—A pluripotent control cell of the vessel wall. Basel, Switzerland: Karger; 1983: 13–28.Google Scholar
- 4.Gajdusek, C. M.; Schwartz, S. M. Technique for cloning bovine aortic endothelial cells. In Vitro 19:394–402; 1983.PubMedCrossRefGoogle Scholar
- 5.Gimbrone, M. A.; Cotran, R. S.; Folkman, J. Human vascular endothelial cells in culture—growth and DNA synthesis. J. Cell Biol. 60:673–684; 1974.PubMedCrossRefGoogle Scholar
- 6.Gospodarowicz D.; Moran J.; Braun, D.; Brodwell C. Clonal growth of bovine vascular endothelial cells: Fibroblast growth factor as a survival agent. Proc. Natl. Acad. Sci. USA 73:4120–4124; 1976.PubMedCrossRefGoogle Scholar
- 7.Holthofer, H.; Virtanen, I.; Kariniemi, A. L.; Hormia, M.; Linder, E.; Miettinen, A.Ulex europeaus I lectin as a marker for vascular endothelium in human tissues. Lab Invest. 47: 60–66; 1982.PubMedGoogle Scholar
- 8.Pober, J. S.; Gimbrone, Jr., M. A. Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: Demonstration by monoclonal antibody binding and immunoprecipitation. Proc. Natl. Acad. Sci. USA 79: 6641–6645; 1982.PubMedCrossRefGoogle Scholar
- 9.Thiolo-Korner, D. G. S.; Heinrich, D.; Temme, H. Endothelial cells in culture. In: Thilo-Korner, D. G. S.; Freshney, R. I., eds. The endothelial cell—A pluripotent control cell of the vessel wall. Basel, Switzerland: Karger; 1983; 158–202.Google Scholar
- 10.Thornton, S. C.; Mueller, S. N.; Levine, E. M. Human endothelial cells: Use of heparin in cloning and long-term serial cultivation. Science 222:623–625; 1983.PubMedCrossRefGoogle Scholar
- 11.Watanabe, T. Electron microscopy of cell surfaces of paramecium caudatum stained with Ruthenium red. Tissue Cell 13:1–7; 1981.PubMedCrossRefGoogle Scholar