A new cell line, designated BCIRL-HA-AM1, derived from ovaries of female pupas ofHeliothis armigera (Hbn.) has been established. Both oval and round cells were found in BCIRL-HA-AM1. Chromosomes were typical of lepidoptera (i.e. short condensed rods and spheres; a majority of cells were polyploid). TheH. armigera line was distinguished fromH. zea andH. virescens cell lines as well as two other noctuid cell lines,Trichoplusia ni (Hübner) andSpodoptera frugiperda (J. E. Smith), by use of isoelectric focusing and the enzyme phosphoglucoisomerase. This cell line would not replicate (i.e. produce polyhedral inclusion bodies or show an increase in virus titer) the single-embedded nuclear polyhedrosis virus (NPV) ofH. zea nor the multiple-embedded NPV ofH. armigera orAutographa californica Speyer.
Heliothis armigeracell line morphology isoelectric focusing