In Vitro

, Volume 8, Issue 5, pp 375–378 | Cite as

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

  • Arthur H. Mc Intosh
  • K. Maramorosch
  • C. Rechtoris


An established insect cell line (AC20) from the leafhopperAgallia constricta has been adapted to a mammalian cell culture medium based on the formulation of two commercially available media. The cell population doubling time of the adapted line in this medium is approximately 45 hr at 30°C.

Key words

insect cell line mammalian medium 


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  1. 1.
    Schneider, I. 1967. Insect tissue culture. In: F. H. Wilt and N. K. Wessells (Eds.),Methods in Developmental Biology. Crowell Co., New York, pp. 543–554.Google Scholar
  2. 2.
    Vago, C. 1967. Invertebrate tissue culture. In: K. Maramorosch and H. Koprowski (Eds.).Methods in Virology, Vol. I. Academic Press, New York, pp. 567–602.Google Scholar
  3. 3.
    Brooks, M. A., and T. J. Kurtti. 1971. Insect cell and tissue culture. Annu. Rev. Entomol. 16: 27–52.PubMedCrossRefGoogle Scholar
  4. 4.
    Hirumi, H., and K. Maramorosch. 1971. Cell culture of Hemiptera. In: C. Vago (Ed.).Invertebrate Tissue Culture, Vol. I. Academic Press, New York, pp. 307–339.Google Scholar
  5. 5.
    Marks, E. P. 1970. The action of hormones in insect cell and organ cultures. Gen. Comp. Endocrinol. 15: 280–302.CrossRefGoogle Scholar
  6. 6.
    Vaughn, L. 1971. Cell culture media and methods. In: C. Vago (Ed.),Invertebrate Tissue Culture, Vol. I. Academic Press, New York, pp. 3–40.Google Scholar
  7. 7.
    Kimura, I., and L. M. Black. 1971. Some factors affecting infectivity assays of wound tumor virus on cell monolayers from an insect vector. Virology 46: 266–276.PubMedCrossRefGoogle Scholar
  8. 8.
    Hink, W. F. 1970. Established insect cell line from the cabbage looper,Trichoplusia ni. Nature (London) 226: 226–467.CrossRefGoogle Scholar
  9. 9.
    Hink, W. F., and B. J. Ellis. 1971. Establishment and characterization of two new cell lines (CP-1268 and CP-169) from the codling moth,Carpocapsa pomonella (with a review of culture of cells and tissues from Lepidoptera). Curr. Top. Microbiol. Immunol. 55: 19–28.PubMedGoogle Scholar
  10. 10.
    Hirumi, H., and K. Maramorosch. 1964. Thein vitro cultivation of embryonic leafhopper tissues. Exp. Cell Res. 36: 625–631.PubMedCrossRefGoogle Scholar
  11. 11.
    Hirumi, H. 1972. Morphological characterization of the leafhopper AC20 line cells. Proc. 3rd Internat. Colloq. Invertebrate Tissue Culture, Smolenice, Czechoslovakia, 1971, in press.Google Scholar
  12. 12.
    Morgan, J. F., H. J. Morton, and R. C. Parker. 1950. Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proc. Soc. Exp. Biol. Med. 73: 1–8.PubMedGoogle Scholar
  13. 13.
    Melnick, J. L. 1955. Tissue culture techniques and their application to original isolation, growth, and assay of poliomyelitis and orphan viruses. Ann. N. Y. Acad. Sci. 61: 754–772.PubMedCrossRefGoogle Scholar
  14. 14.
    Merchant, D. L., R. H. Kahn, and W. H. Murphy. 1964.Handbook of Cell and Organ Culture, 2nd ed. Burgess Publishing Co., Minneapolis.Google Scholar
  15. 15.
    Hayflick, L. 1965. Tissue cultures and mycoplasmas. Tex. Rep. Biol. Med. 23: 285–303.PubMedGoogle Scholar
  16. 16.
    Sohi, S. S., and Y. Hayashi. 1971. Metabolism ofAedes aegypti cells grown in vitro. II. Determination of cell viability. In Vitro 7: 146–151.PubMedCrossRefGoogle Scholar
  17. 17.
    Yunker, C. E., and J. Cory. 1969. Colorado tick fever virus: growth in a mosquito cell line. J. Virol. 3: 631–632.PubMedGoogle Scholar

Copyright information

© Tissue Culture Association 1973

Authors and Affiliations

  • Arthur H. Mc Intosh
    • 1
  • K. Maramorosch
    • 1
  • C. Rechtoris
    • 1
  1. 1.Boyce Thompson Institute for Plant ResearchYonkers

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