In Vitro

, Volume 16, Issue 5, pp 446–452 | Cite as

Lymphoid cell line identification and the detection of cross contamination

  • Betty R. Conner
  • Soldano Ferrone
  • Michele A. Pellegrino
  • Ronald Glaser
Article
Received:
Accepted:
  • 23 Downloads

Summary

Four lymphoid cell lines, presumably recent derivatives from non-neoplastic human tonsils, were identified in actuality as established Burkitt, lymphoma and lymphoblastoid cell lines (LCL) when tested by chromosome banding and typing for HLA antigens. Additionally, the morphology, growth characteristics, tumorigenicity, expression of Epstein-Barr virus antigens and surface membrane immunoglobulins (SmIg) of the four lymphoid cell lines confirmed the correct identifications. In determining the possiblity of cross contamination, the most reliable criteria for the identification of lymphoid cell lines were found to be HLA antigenic profile, karyotype, cellular morphology, and growth characteristics.

Key words

lymphoid lines HLA antigens karyotype Burkitt lymphoma 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Gartler, S. M. Genetic markers as tracers in cell cultures. Natl. Cancer Inst. Monogr. 26: 167–195; 1966.Google Scholar
  2. 2.
    Gartler, S. M. Apparent HeLa cell contamination of human heteroploid cell lines. Nature (London) 217: 750–751; 1968.CrossRefGoogle Scholar
  3. 3.
    Nelson-Rees, W. A.; Flandermeyer, R. R.; Hawthorne, P. K. Banded marker chromosomes as indicators in intraspecies cellular contamination. Science 184: 1093–1096; 1974.PubMedCrossRefGoogle Scholar
  4. 4.
    Hinuma, Y.; Konn, M.; Yamaguchi, J.; Wudarski, D. J.; Blakeslee, J. R. Jr.; Grace, J. T. Jr. Immunofluorescence and herpes-type particles in the P3HR-1 Burkitt lymphoma cell line. J. Virol. 1: 1045–1051; 1967.PubMedGoogle Scholar
  5. 5.
    Epstein, M. A.; Achong, B. G.; Barr, Y. M.; Zajac, B.; Henle, G.; Henle, W. Morphological and biological investigation on cultured Burkitt tumor lymphoblasts (strain Raji). J. Natl. Cancer Inst. 37: 547–559; 1966.PubMedGoogle Scholar
  6. 6.
    Miller, G.; Shope, T.; Lisco, H.; Stitt, D.; Lipman, M. Epstein-Barr virus: Transformation, cytopathic changes and viral antigens in squirrel monkey and marmoset leucocytes. Proc. Natl. Acad. Sci. U.S.A. 69: 383–387; 1972.PubMedCrossRefGoogle Scholar
  7. 7.
    Hiracki, S.; Miyoshi, I.; Masuji, H.; Kubonishi, I.; Matsuda, Y.; Nakayama, T.; Kishimoto, H.; Chen, P.; Kimura, I. Establishment of an Epstein-Barr virus-determined nuclear antigenegative human B-cell line from acute lympho-blastic leukemia J. Natl. Cancer Inst. 59: 93–94; 1977.Google Scholar
  8. 8.
    Klein, E.; Klein, G.; Nadkarni, J. S.; Nadkarni, J. J.; Wigzell, H.; Clifford, P. Surface IgM kappa specificity on a Burkitt lymphoma cellin vivo and in derived culture lines. Cancer Res. 28: 1300–1310; 1968.PubMedGoogle Scholar
  9. 9.
    Matsuoka, Y.; Moore, G. E.; Yagi, Y.; Pressman, D. Production of free light chains of imm noglobulin by a hematopoietic cell line derived from a patient with multiple myeloma. Proc. Soc. Exp. Biol. Med. 125: 1246–1250; 1967.PubMedGoogle Scholar
  10. 10.
    Veltri, R. W.; Heyl, L. W.; Sprinkle, P. M. Epstein-Barr virus genome carrying lymphocyte supopulations of human palatine tonsils. Proc. Soc. Exp. Biol. Med. 156: 282–286; 1977.PubMedGoogle Scholar
  11. 11.
    Veltri, R. W.; McClung, J. E.; Sprinkle, P. M. EBV antigens in lymphocytes of patients with exudative tonsillitis, infectious mononucleosis and Hodgkin's disease. Int. J. Cancer 21: 683–687; 1978.PubMedCrossRefGoogle Scholar
  12. 12.
    Moorhead, P. S.; Nowell, P. C.; Mellman, W. J.; Battips, D. M.; Hungerford, D. A. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp. Cell Res. 20: 613–616; 1960.PubMedCrossRefGoogle Scholar
  13. 13.
    Caspersson, T.; Zech, L.; Johansson, C. Analysis of human metaphase chromosome set by aid of DNA-binding fluorescing agents. Exp. Cell Res. 62: 490–492; 1970.PubMedCrossRefGoogle Scholar
  14. 14.
    Pellegrino, M. A.; Ferrone, S.; Pellogrino, A. A simple microabsorption technique for HL-A typing. Proc. Soc. Exp. Biol. Med. 139: 484–488; 1972.PubMedGoogle Scholar
  15. 15.
    Giovanella, B. C.; Stehlin, J. S.; Williams, L. S. Heterotransplantation of human malignant tumors in “nude” thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. J. Natl. Cancer Inst. 52: 921–930; 1974.PubMedGoogle Scholar
  16. 16.
    Reedman, B. M.; Klein, G. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producar and non-producer lymphoblastoid cell lines. Int. J. Cancer 11: 499–520; 1973.PubMedCrossRefGoogle Scholar
  17. 17.
    Henle, G.; Henle, W. Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bacteriol. 91: 1248–1256; 1966.PubMedGoogle Scholar
  18. 18.
    Moss, D. J.; Pope, J. H. Assay of the infectivity of Epstein-Barr virus by transformation of human leukocytesin vitro. J. Gen. Virol. 17: 233–236; 1972.PubMedCrossRefGoogle Scholar
  19. 19.
    Henle, W.; Henle, G.; Zajac, B. A.; Pearson, G.; Waubke, R.; and Scriba, M. Differential reactivity of human serums with early antigens induced by Epstein-Barr virus. Science 169: 188–190; 1970.PubMedCrossRefGoogle Scholar
  20. 20.
    Nonyama, M.; Pagano, J. Detection of Epstein-Barr viral genome in non-productive cells. Nature (London) 233: 103–106; 1971.Google Scholar
  21. 21.
    Winchester, R. J. Techniques of surface immuno-fluorescence applied to the analysis of the lymphocyte. Bloom, B. R.; David, J. R. eds.In vitro methods in cell mediated and tumor immunology. New York: Academic Press; 1976: 171–186.Google Scholar
  22. 22.
    Nilsson, K.; Ponten, J. Classification and biological nature of established human hematopoietic cell lines. Int. J. Cancer 15: 321–341; 1975.PubMedCrossRefGoogle Scholar
  23. 23.
    Jarvis, J. E.; Ball, G.; Rickinson, A. B.; Epstein, M. A. Cytogenetic studies on human lymphoblastoid cell lines from Burkitt's lymphoma and other sources. Int. J. Cancer 14: 716–721; 1974.PubMedCrossRefGoogle Scholar
  24. 24.
    Manalov, G.; Manalova, Y. Marker band in one chromosome 14 from Burkitt lymphomas. Nature (London) 237: 33–34; 1972.CrossRefGoogle Scholar
  25. 25.
    Zech, L.; Hagland, U.; Nilsson, K.; Klein, G. Characteristic chromosomal abnormalities in biopsies and lymphoid cell lines from patients with Burkitt and non-Burkitt lymphomas. Int. J. Cancer 17: 47–56; 1976.PubMedCrossRefGoogle Scholar
  26. 26.
    Rogentine, G. N. Jr.; Gerber, P. HL-A antigens of human lymphoid cells in long-term culture. Transplantation 8: 28–37; 1969.PubMedCrossRefGoogle Scholar
  27. 27.
    Reisfeld, R. A.; Pellegrino, M. A.; Dierich, M. P.; Ferrone, S. Cultured human lymphoid cells: Tools for the biological and molecular characterization of antigens and their recognition systems. In Vitro 11: 173–185; 1975.PubMedCrossRefGoogle Scholar
  28. 28.
    Nilsson, K.; Giovanella, B. C.; Stehlin, J. S.; Klein, G. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337–344; 1977.PubMedCrossRefGoogle Scholar
  29. 29.
    Pagano, J. S.; Huang, C.; Huang, Y. Epstein-Barr virus genome in infectious mononucleosis. Nature (London) 263: 787–789; 1976.CrossRefGoogle Scholar
  30. 30.
    Bechet, J. M.; Fialkow, P. J.; Nilsson, K.; Klein, G.; Singh, S. Immunoglobulin synthesis and glucose-6-phosphate dehydrogenase as cell markers in human lymphoblastoid cells. Exp. Cell Res. 89: 275–282; 1975.CrossRefGoogle Scholar
  31. 31.
    Mittal, K. K.; Mickey, M. R.; Singal, D. P.; Terasaki, P. I. Serotyping for homotransplantation XVII. Refinement of microdroplet lymphocyte cytotoxicity test. Transplantation 6: 913–927; 1968.PubMedCrossRefGoogle Scholar
  32. 32.
    Ferrone, S.; Tosi, R. M.; Centis, D. Anticomplementary factors affecting the lymphocytotoxicity test. Histocompatibility testing 1967. Curtoni, E. S.; Mattiuz, P. L.; Tosi, R. M. eds. Copenhagen: Munksgaard; 1967: 357–364.Google Scholar
  33. 33.
    Ferrone, S.; Pellegrino, M. A.; Reisfeld, R. A. A biologic and chemprofile of histocompatibility antigens. Sela, M. ed. The antigens; vol. III. New York: Academic Press; 1975: 362–447.Google Scholar
  34. 34.
    Walford, R. L.; Shanbrom, E.; Troup, G. M.; Zeller, E.; Ackerman, B. 1967. Lymphocyte grouping with defined antisera. Histocompatibility testing. Curtoni, E. S.; Mattiuz, P. L.; Tosi, R. M. eds. Copenhagen: Munksgaard; 1967: 357–364.Google Scholar
  35. 35.
    Traul, K. A.; Stephens, R.; Gerber, P.; Peterson, W. D. Productive Epstein-Barr viral infection of the human lymphoblastoid cell line 6410 with release of early antigen inducing and transforming virus. Int. J. Cancer 20: 247–255; 1977.PubMedCrossRefGoogle Scholar
  36. 36.
    Huang, C. C.; Minowada, J. Differential effects of infection with herpes simplex virus on the chromosomes of human hepatopoietic cell lines. Cancer Res 32: 1218–1225; 1972.PubMedGoogle Scholar
  37. 37.
    Minowada, J.; Nonoyama, M.; Moore, G. E.; Rauch, A. M.; Pagano, J. The presence of the Epstein-Barr viral genome in human lymphoblastoid B-cell lines and its absence in a myeloma cell line. Cancer Res. 34: 1898–1903; 1974.PubMedGoogle Scholar

Copyright information

© Tissue Culture Association 1980

Authors and Affiliations

  • Betty R. Conner
    • 1
  • Soldano Ferrone
    • 2
  • Michele A. Pellegrino
    • 2
  • Ronald Glaser
    • 3
  1. 1.Department of MicrobiologyThe Pennsylvania State University College of MedicineHershey
  2. 2.Scripps Clinic and Research FoundationLa Jolla
  3. 3.Department of Medical Microbiology and Comprehensive Cancer CenterThe Ohio State University College of MedicineColumbus

Personalised recommendations