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In Vitro

, Volume 19, Issue 11, pp 825–832 | Cite as

Development of a medium for in vitro culture of oocytes from the polychaeteNereis virens

  • A. E. Heacox
  • A. Fischer
  • H. -R. Frangenberg
Article

Summary

A culture medium was developed that allowed the maintenance and growth of oocytes from the marine polychaeteNereis virens. The ionic composition of the basic salt solution and the medium was similar to that ofNereis coelomic fluid and had a total osmolarity of 920 mOsm. Longevity and the growth of cultured oocytes were investigated with respect to the presence of amino acids andNereis vitellin in the medium. Some oocytes survived for up to 6 wk. In vitro growth occurred at a rate of about 1 μm diam/d (15°C) and cultured cells seemed morphologically and ultrastructurally normal.

Key words

cell culture oocyte marine polychaete 

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Copyright information

© Tissue Culture Association, Inc. 1983

Authors and Affiliations

  • A. E. Heacox
    • 1
  • A. Fischer
    • 1
  • H. -R. Frangenberg
    • 1
  1. 1.Zoological InstituteUniversity of KölnKöln 41West Germany (FRG)

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