In Vitro

, Volume 14, Issue 12, pp 966–980 | Cite as

Selection and characterization of bovine aortic endothelial cells

  • Stephen M. Schwartz

Summary

This paper reports techniques for isolation, selection and long-term passage of bovine aortic endothelium (BAE). A [3H]thymidine-selection technique was developed to limit overgrowth of cultures by contaminating smooth-muscle cells. The resulting cultures could be passaged for a replicative life span of 35 to 40 doublings and maintained a stable, normal karyotype throughout this period. Despite the fact that these cultures reached a stable monolayer with density-inhibited growth state, postconfluent cells showed focal areas of a second growth pattern called “sprouting.” This was seen only when cultures were maintained at high densities for periods of 1 to 2 weeks. Ultrastructural analysis, as well as immunofluorescence studies with markers for endothelial cells (factor VIII) and smooth-muscle cells (actin), indicates that this phenomenon is not due to overgrowth of a residual population of smooth-muscle cells, but may represent a second growth pattern of the endothelial cells themselves.

Key words

thymidine selection endothelium contact inhibition replicative life span 

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Copyright information

© Tissue Culture Association 1978

Authors and Affiliations

  • Stephen M. Schwartz
    • 1
  1. 1.Deparment of PathologyUniversity of WashingtonSeattle

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