Multiple differentiated functions in an unusual clonal strain of hepatoma cells
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MH1C1 cells produce serum albumin and secrete it into the culture medium. The rate of production of albumin is stimulated 3- to 12-fold by adding hydrocortisone to the medium.
The ninth component of complement (C9) is synthesized by MH1C1 cells, a finding which gives evidence that this trace complement component may be a liver product. The synthesis of C9 is not stimulated by hydrocortisone.
Testosterone is metabolized by the MH1C1 cells by pathways which are very similar to, or the same as, those found in normal liver. Evidence for the following testosterone metabolizing enzymes has been obtained: 17β-hydroxysteroid dehydrogenase, Δ4-3-ketosteroid 5α-reductase, 6β-, 7α-, and 16β-hydroxylases, urindine diphosphate glucuronyl transferase, and 17β-hydroxysteroid sulfotransferase.
MH1C1 cells produce tyrosine aminotransferase and respond to hydrocortisone by rapidly increasing the activity of this enzyme.
Multiple differentiated functions which are responsive to exogenous signals lead us to conclude that the MH1C1 cells are a useful model for studying the integration of control mechanisms within a single type of cell.
KeywordsHydrocortisone Testosterone Cell Protein Population Doubling Time Tyrosine Aminotransferase
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