In Vitro

, Volume 9, Issue 5, pp 303–310 | Cite as

Divergen properties of two human lymphocytic cell lines isolated from a single specimen of peripheral blood

  • Herbert Lazarus
  • E. F. Barell
  • S. Oppenheim
  • Awtar Krishan
Article

Summary

Two lymphocytic cell lines were obtained from a single specimen of peripheral blood buffy coat from a patient with acute lymphoblastic leukemia who concurrently developed classical infectious mononucleosis. These two cell lines exhibit distinguishing differences in culture in parallel with reviously described differences in the cells when implanted into eonatal Syrian hamsters. These observations suggest that the cell lines were derived from two pre-existing different classes or “stelines” of cells—one of which resembles other cell lines derived from patients with acute lymphoblastic leukemia, whereas the other resembles cell lines derived from other, patients with infecttious mononucleosis.

Keywords

Acute Lymphoblastic Leukemia Buffy Coat Infectious Mononucleosis Lymphocytic Cell Ketobutyrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Benyesh-Melnick, M., D. J. Fernbach, and R. T. Lewis. 1963. Studies on human leukmia. I. Spontaneous lymphoblastoid transformation of fibroblastic bone marrow cultures derived from leukemic and nonleukemic children. J. Natl. Cancer Inst. 31: 1311–1325.PubMedGoogle Scholar
  2. 2.
    Foley, G. E, H. Lazarus, S. Farber, B. G. Uzman, B. A. Boone, and R. E. McCarthy. 1965. Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522–529.PubMedCrossRefGoogle Scholar
  3. 3.
    Jensen, E. M., W. Korol, S. L. Dittmar, and T. J. Medrek. 1967. Virus containing lymphocyte cultures from cancer patients. J. Natl. Cancer Inst. 39: 745–754.PubMedGoogle Scholar
  4. 4.
    Moore, G. E., J. T. Gracc, Jr., P. Citron, R. Gerner, and A. Burns. 1966. Lekocytecultures of patients with, leukemia and lymphomas. N. Y. State J. Med. 66: 2757–2764.PubMedGoogle Scholar
  5. 5.
    Lazarus, H., and G. E. Foley. 1972. Cultivation of hematopoietic cells. In: G. H. Rothblat. and V. J. Cristofalo (Eds.),Growth, Nutrition and Metabolism of Cells in Culture. Volume II, Chapter 5. Academic Press, New York, pp. 170–212.Google Scholar
  6. 6.
    Adams, R. A., L. Pothier, A. Flowers, H. Lazarus, S. Farber, and G. E. Foley. 1970. The question of stem-lines in human acute leukemia: Comparison of cells islated in vitro and in vivo from a patient with acute lymphoblastic leukemia. Expt. Cell Res. 62: 5–10.CrossRefGoogle Scholar
  7. 7.
    Adams, R. A., A. Flowers, and B. J. Davis. 1968. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121–1125.PubMedGoogle Scholar
  8. 8.
    Foley, G. E., H. Lazarus, S. Farber, B. G. Uzman, and R. A. Adams. 1968. Studies on human leukemic cells in vitro. In:The Proliferation and Spread of Neoplastic Cells. 21st Annual Symposium on Fundamental Cancer Research, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, TX. The Williams & Wilkins Co., Baltimore, pp. 65–97.Google Scholar
  9. 9.
    Eagle, H. 1959. Amino acid metabolism in mammalian cell cultures. Science 30: 432–437.CrossRefGoogle Scholar
  10. 10.
    Uzman, B. G., G. E. Foley, S. Farber, and H. Lazarus. 1966. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term cuture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725–1742.PubMedCrossRefGoogle Scholar
  11. 11.
    Friedkin, M., and D. Roberts. 1954. The enzymatic synthesis of nucleotides. I. Thymidine phosphorylase in mammalian tissue. J. Biol. Chem. 207: 245–256.PubMedGoogle Scholar
  12. 12.
    Marsh, J. C., and S. Perr. 1964. Thyidine catabolism by normal and leukemic human leukocytes. J. Clin. Invest. 43: 267–278.PubMedCrossRefGoogle Scholar
  13. 13.
    Friedemann, T. E., and G. E. Haugen. 1943. Pyruvic acid. II. The determination of keto acids in blood and urine. J. Biol. Chem. 147: 415–442.Google Scholar
  14. 14.
    Sayre, F. W., and D. M. Greenberg. 1956. Purification and properties of serine and threonine dehydrases. J. Biol. Chem. 220: 787–799.PubMedGoogle Scholar
  15. 15.
    Matsuo, Y., and D. M. Greenberg. 1958. A crystalline enzyme that cleaves homoserine and cystathionine. I. Isolation procedure and some physicochemical properties. J. Biol. Chem. 230: 545–560.PubMedGoogle Scholar
  16. 16.
    Oyama, V. I., and H. Eagle. 1956. Measurment of cell growth in tissue culture with a pheno regent (Folin-Ciocalteau). Proc. Soc. Expt. Biol. Med. 91: 305–307.Google Scholar
  17. 17.
    Uzman, B. G., and A. Krishan. 1969. Virus or virus-derived aggregates in cells cultured from leukemia and infectious mononucleosis. Proc. Am. Assoc. Cancer Res. 10: 95.Google Scholar
  18. 18.
    Moses, H. L., P. R. Glade, J. A. Kasel, A. S. Rosenthal, Y. Hirshaut, and L. N. Chessin. 1968. Infectious mononucleosis: Detection of herpes-like virus and reticular aggregates of small cytoplasmic particles in cotinuous lymphoid cell lines derived from peripheral blood. Proc. Natl. Acad. Sci. U.S.A. 60: 489–496.PubMedCrossRefGoogle Scholar
  19. 19.
    Chandra, S., G. E. Moore, and P. M. Brandt. 1968. Similarity between leukocyte cultures from cancerous and noncancerous human subjects: An electronmicroscopic study. Cancer Res. 28: 1982–1989.PubMedGoogle Scholar
  20. 20.
    Uzman, B. G., H. Saito, and M. Kasac. 1971. Tubular arrays in the endoplasmic reticulum in human tumor cells. Lab. Invest. 24: 492–498.PubMedGoogle Scholar
  21. 21.
    Schaff, Z., U. Heine, and A. J. Dalton. 1972. Ultramorphological and ultracytochemical studies on tubuloreticular structures in lymphoid cells. Cancer Res. 32: 2696–2706.PubMedGoogle Scholar
  22. 22.
    Foley, G. E., E. F. Barell, R. A. Adams, and H. Lazarus. 1969. Nutritonal requirements of human leukemic cells: Cystine requirements of diploid cell lines and their heteroploid variants. Exp. Cell Res. 57: 129–133.PubMedCrossRefGoogle Scholar
  23. 23.
    Schachtschabel, D. O., H. Lazarus, S. Farber, and G. E. Foley. 1966. Sensitivty of cultured human lymphoblasts (CCRF-CEM cells) to inhibition by thymidine. Expt. Cell Res. 43: 512–514.CrossRefGoogle Scholar
  24. 24.
    Vaughan, M. H., and M. W. Steele. 1971. Differential sensitivity of human normal and malignant cells to 8-azahypoxanthine in vitro. Exp. Cell Res. 69: 92–96.PubMedCrossRefGoogle Scholar
  25. 25.
    Krishan, A., and R. Ray-Chaudhuri. 1970. Chromosome studies of cell lines and tumors derived from a single specimen of human leukemic blood by cell culture and heterotransplantation. Cancer Res. 30: 2012–2016.PubMedGoogle Scholar
  26. 26.
    Adams, R. A., G. E. Foley, B. G. Uzman, S. Farber, H. Lazarus, and L. Kleinman. 1967. Leukemia: Serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772–783.PubMedGoogle Scholar
  27. 27.
    Adams, R. A., G. E. Foley, H. Lazarus, S. Oppenheim, and A. Babcock. 1970. Antilymphocyte serum stimulation of immunoglobulin biosynthesis in cultured Burkitt's tumor (EB3) cells. Fed. Proc. 29: 301.Google Scholar
  28. 28.
    Lazarus, H., S. Oppenheim, and G. E. Foley. To be published.Google Scholar
  29. 29.
    Lazarus, H., S. Oppenheim, E. F. Barell, and G. E. Foley. To be published.Google Scholar
  30. 30.
    Tanigaki, N., Y. Yagi, G. E. Moore, and D. Pressman. 1966. Immunoglobulin production in human leukemia cell lines. J. Immunol. 97: 634–646.PubMedGoogle Scholar

Copyright information

© Tissue Culture Association 1974

Authors and Affiliations

  • Herbert Lazarus
    • 1
    • 2
  • E. F. Barell
    • 1
    • 2
  • S. Oppenheim
    • 1
    • 2
  • Awtar Krishan
    • 1
    • 2
  1. 1.The Children's Cancer Research FoundationHarvard Medical SchoolBoston
  2. 2.Department of PathologyHarvard Medical SchoolBoston

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