In Vitro

, Volume 13, Issue 1, pp 41–48 | Cite as

Organogenesis in vitro cultures of embryonic shoots ofAbies balsamea (balsam fir)

  • J. M. Bonga


Embryonic shoots of 15- to 20-year-oldAbies balsamea (balsam fir) trees were soaked in (a) water for 15 min or 24 hr and (b) water with 1000 mg per l indolebutyric acid (IBA), N-dimethylaminosuccinamic acid (Alar-85), or 1-phenyl-3-methyl-5-pyrazolone (PPZ), singly or in combination with 100 mg per 1 caffeic acid, for 15 min. After the soaking, the embryonic shoots were transferred to a nutrient medium. Nonsoaked (control) embryonic shoots elongated and often formed a basal callus but never showed organogenesis. The soaked embryonic shoots formed new apical buds, with or without bud scales, adventitious dwarf needles or shoots, and root- and embryo-like structures. One of the embryos germinated and formed an irregular shoot. No differences were found between the various soak treatments, except that the 15-min water soak was ineffective. The 24-hr water soak was as effective as the 15-min growth regulator treatments.

Key words

Abies balsamea organogenesis vegetative propagation 


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Copyright information

© Tissue Culture Association 1977

Authors and Affiliations

  • J. M. Bonga
    • 1
  1. 1.Maritimes Forest Research CentreCanadian Forestry Service, Department of the EnvironmentFredericton

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