Response of mammalian cells to controlled growth rates in steady-state continuous culture
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Mouse LS cells grow in completely mixed steady-state continuous suspension (“chemostat”) culture in defined medium.
The steady-state concentration of cells is maximal at a dilution rate of 0.30 to 0.35 day−1.
Glucose can act as the limiting substrate for LS cells under chemostat conditions.
The glucose oxidation rate per cell does not vary with dilution rate.
Maintenance energy is 19 picomoles of ATP per cell per day. Growth energy is 22 picomoles of ATP per cell.
Slowly growing cells contain more protein and less RNA per cell than rapidly growing cells.
The “efficiency” of protein synthesis decreases in slowly growing cells, in which a lower proportion of ribosomes is present in the form of polysomes or ribosomal subunits.
Newly-made 18S RNA appears early in the cytoplasm of rapidly growing cells, but is greatly delayed in slowly growing cells.
Pulsed additions of a limiting substrate to steady-state populations may lead to synchronized cells that have a controlled interdivision time. Hence chemostat cultures may be used to investigate the interdependence of events in the cell cycle.
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