Summary
The history and characteristics of two cell lines developed from primary explants of pupal tissue from the insect,Spodoptera frugiperda (J. E. Smith), are described. One cell line, IPLB-SF-21, was developed with hemolymph-supplemented medium and has been maintained continuously on the medium. The second cell line, IPLB-SF-1254, was developed with a medium containing a combination of vertebrate sera plus hemolymph and was adapted to hemolymph-free medium at the 6th passage. The IPLB-SF-1254 line is 36 hr. The chromosomal morphology and distribution was typical of other lepidopteran cell lines. Serological studies showed that both cell lines have at least one antigen which also is common to tissue antigens from pupae ofSpodoptera frugiperda.
References
Hink, W. F. 1976. A compilation of invertebrate cell lines and culture media. In: K. Maramorosch (Ed.),Invertebrate Tissue Culture Research Applications. Academic Press, New York, pp. 319–369.
Greene, A. E., J. Charney, W. W. Nichols, and L. L. Coriell. 1972. Species identity of insect cells. In Vitro 7: 313–322.
Goodwin, R. H. 1975. Insect cell culture: improved media and methods for initiating attached cell lines from the Lepidoptera. In Vitro 11: 369–378.
Yunker, C. E., J. L. Vaughn, and J. Cory. 1967. Adaptation of an insect cell line (Grace’sAntheraea cells) to medium free of insect hemolymph. Science 155: 1565–1566.
Hink, W. F. 1970. Established insect cell line from the cabbage looper,Trichoplusia ni. Nature 226: 466–467.
Hink, W. F., and C. M. Ignoffo. 1970. Establishment of a new cell line (IMC-HZ-1) from ovaries of cotton bollworm moths,Heliothis zea (Boddie). Exp. Cell Res. 60: 307–309.
Hink, W. F., and B. J. Ellis. 1971. Establishment and characterization of two new cell lines (CP-1268 and CP-169) from the codling moth,Carpocapsa pomonella (with a review of culture of cells and tissues from Lepidoptera). In: E. Weiss (Ed.),Arthropod Cell Cultures and Their Application to the Study of Viruses. Curr. Top. Microbiol. Immunol. 55: 19–28.
Sohi, S. S. 1973.In vitro cultivation of larval tissues ofChoristoneura fumiferana (Clemens.) (Lepidoptera: Tortricidae). In: J. Rehacek, D. Blaskovic, and W. F. Hink (Eds.),Proceedings International Colloquium Invertebrate Tissue Culture, 1971, Smolenice, Czech. Slovak Acad. Sci. Publ. House, Bratislava, pp. 75–92.
Eide, P. E., J. M. Caldwell, and E. P. Marks. 1975. Establishment of two cell lines from embryonic tissue of the tobacco hornworm,Manduca sexta (L.). In Vitro 11: 395–399.
Knudson, D. L., and T. W. Tinsley. 1974. Replication of a nuclear polyhedrosis virus in a continuous cell culture ofSpodoptera frugiperda: purification, assay of infectivity, and growth characteristics of the virus. J. Virol. 14: 934–944.
Goodwin, R. H., J. L. Vaughn, J. R. Adams, and S. J. Louloudes. 1973. The influence of insect cell lines and tissue culture media on Baculovirus polyhedra production. Misc. Publ. Entomol. Soc. Am. 9: 66–72.
Gardiner, G. R., and H. Stockdale. 1975. Two tissue culture media for production of lepidopteran cells and nuclear polyhedrosis viruses. J. Invertebr. Pathol. 25: 363–370.
McIntosh, A. H., and S. B. Padhi. 1976. In vitro and in vivo comparative studies of several nuclear polyhedrosis viruses (NPVs) by neutralization, immunofluorescence and polyacrylamide gel electrophoresis. In: E. Kurstak, and K. Maramorosch (Eds.),Invertebrate Tissue Culture: Applications in Medicine, Biology, and Agriculture. Academic Press, New York, pp. 331–338.
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At the time this work was done, Ronald H. Goodwin was a Postdoctoral Research Fellow sponsored by the National Academy of Sciences.
Mention of a proprietary product does not constitute endorsement by the U.S. Department of Agriculture.
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Vaughn, J.L., Goodwin, R.H., Tompkins, G.J. et al. The establishment of two cell lines from the insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro 13, 213–217 (1977). https://doi.org/10.1007/BF02615077
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DOI: https://doi.org/10.1007/BF02615077