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Current Microbiology

, Volume 2, Issue 2, pp 123–126 | Cite as

Breakdown of Douglas-fir phloem by a lignocellulose-degradingStreptomyces

  • John B. Sutherland
  • Robert A. Blanchette
  • Don L. Crawford
  • Anthony L. PomettoIII
Article

Abstract

An actinomycete,Streptomyces flavovirens, was shown to decay the intact cell walls of Douglas-fir phloem. Disks that had been cut from the inner bark were autoclaved, leached with sterile water, and inoculated withS. flavovirens. Decay produced by the actinomycete was observed by scanning electron microscopy, and the residual phloem was analyzed for weight loss and changes in carbohydrate and lignin content. After growth ofS. flavovirens for 12 weeks, inoculated disks had lost 47% of the leached dry weight, whereas sterile control disks had lost only 12%. The bulk of the material degraded by the actinomycete was carbohydrate. Scanning electron micrographs of decayed phloem disks showed irregular cavities in the cell walls of parenchyma and sclereids, with actinomycete hyphae penetrating these cavities.

Keywords

Lignin Streptomyces Bark Current Microbiology Control Disk 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1979

Authors and Affiliations

  • John B. Sutherland
    • 1
  • Robert A. Blanchette
    • 2
  • Don L. Crawford
    • 1
  • Anthony L. PomettoIII
    • 1
  1. 1.Department of Bacteriology and Biochemistry, Idaho Agricultural Experiment StationUniversity of IdahoMoscowUSA
  2. 2.Department of Plant Pathology, Washington Agricultural Experiment StationWashington State UniversityPullmanUSA

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