Current Microbiology

, Volume 4, Issue 2, pp 111–116 | Cite as

Legionella bozemanii sp. nov. andLegionella dumoffii sp. nov.: Classification of two additional species ofLegionella associated with human pneumonia

  • Don J. Brenner
  • Arnold G. Steigerwalt
  • G. W. Gorman
  • Robert E. Weaver
  • James C. Feeley
  • L. G. Cordes
  • Hazel W. Wilkinson
  • Charlotte Patton
  • Berenice M. Thomason
  • Karen R. Lewallen Sasseville
Article

Abstract

Deoxyribonucleic acid (DNA) relatedness was used to distinguish strains ofLegionella-like organisms (LLO) fromLegionella pneumophila. Two of these LLO strains, WIGA and MI 15, showed sufficient DNA relatedness to one another to be classified in the same species. The nameLegionella bozemanii species nova is proposed for this new species. The type strain ofL. bozemanii is WIGA (=ATCC 33217) Two other LLO strains, NY 23 and Tex-KL, were shown to represent a new species. The nameLegionella dumoffii species nova is proposed for this species. The type strain ofL. dumoffii is NY 23 (=ATCC 33279). These two species joinL. pneumophila andL. micdadei in the genusLegionella.

Keywords

Current Microbiology Cellular Fatty Acid Composition Slide Agglutination Test Disease Bacterium Postmortem Lung 

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Literature Cited

  1. 1.
    Brenner, D. J., Steigerwalt, A. G., McDade, J. M. 1979. Classification of the Legionnaires’ disease bacterium:Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Annals of Internal Medicine90:656–658.PubMedGoogle Scholar
  2. 2.
    Brenner, D. J., Steigerwalt, A. G., Weaver, R. E., McDade, J. E., Feeley, J. C., Mandel, M., 1978. Classification of the Legionnaires’ disease bacterium. An interim report. Current Microbiology1:71–75.Google Scholar
  3. 3.
    Bozeman, F. M., Humphries, J. W., Campbell, J. A. 1968. A new group of rickettsia-like agents recovered from guinea pigs. Acta Virologica12:87–93.PubMedGoogle Scholar
  4. 4.
    Cordes, L. G., Wilkinson, H. W., Gorman, G. W., Files, B. J., Fraser, D. W. 1979. AtypicalLegionella-like organisms: Fastidious water-associated bacteria pathogenic for man. Lancetii:927–930.CrossRefGoogle Scholar
  5. 5.
    Feeley, J. C., Gibson, R. J., Gorman, G. W., Langford, N. C., Rasheed, J. K., Mackel, D. C., Baine, W. B. 1979. Charcoal-yeast extract agar Primary isolation medium forLegionella pneumophila. Journal of Clinical Microbiology10:437–441.PubMedGoogle Scholar
  6. 6.
    Feeley, J. C., Gorman, G. W., Weaver, R. E., Mackel, D. C., Smith, W. H. 1978. Primary isolation media for the Legionaires disease bacterium. Journal of Clinical Microbiology8:320–325.PubMedGoogle Scholar
  7. 7.
    Hébert, G. A., Moss, C. W., McDougal, L. K., Bozeman, F. M., McKinney, R. M., Brenner, D. J. 1980. The rickettsia-like organisms TATLOCK (1943) and HEBA (1959): Bacteria phenotypically similar to but genetically distinct fromLegionella pneumophila and the WIGA bacterium. Annals of Internal Medicine92:45–52.PubMedGoogle Scholar
  8. 8.
    Hébert, G. A., Steigerwalt, A. G., Brenner, D. J. 1980.Legionella micdadei species nova: Classification of a third species ofLegionella associated with human pneumonia. Current Microbiology3:255–257.CrossRefGoogle Scholar
  9. 9.
    Jackson, E. B., Crocker, T. T., Smadel, J. E. 1952. Studies on two rickettsia-like agents probably isolated from guinea pigs. Bacteriological Proceedings1952: 119.Google Scholar
  10. 10.
    Lewallen, K. R., McKinney, R. M., Brenner, D. J., Moss, C. W., Dail, D. H., Thomason, B. M., Bright, R. A. 1979. A newly identified bacterium phenotypically resembling, but genetically distinct from,Legionella pneumophila: An isolate in a case of pneumonia. Annals of Internal Medicine91:831–834.PubMedGoogle Scholar
  11. 11.
    McDade, J. E., Brenner, D. J., Bozeman, F. M. 1979. Legionnaires’ disease bacterium isolated in 1947. Annals of Internal Medicine90:659–661.PubMedGoogle Scholar
  12. 12.
    McKinney, R. M., Thacker, L., Harris, P. P., Lewallen, K. R., Hébert, G. A., Edelstein, P. H., Thomason, B. M. 1979. Four serogroups of Legionnaires’ disease bacteria defined by direct immunofluorescence. Annals of Internal Medicine90:659–661.Google Scholar
  13. 13.
    Moss, C. W., Dees, S. B. 1979. Cellular fatty acid composition of WIGA, a rickettsia-like agent similar to the Legionnaires’ disease bacterium. Journal of Clinical Microbiology10:390–391.PubMedGoogle Scholar
  14. 14.
    Moss, C. W., Weaver, R. E., Dees, S. B., Cherry, W. B. 1977. Cellular fatty acid composition of isolates from Legionnaires disease. Journal of Clinical Microbiology6:140–143.PubMedGoogle Scholar
  15. 15.
    Pine, L., George, J. R., Reeves, M. W., Harrell, W. K. 1979. Physiology: Characteristics of the Legionnaires’ disease bacterium in semisynthetic and chemically defined liquid media, pp. 27–40. In: Jones, G. L., Hébert, G. A. (eds.), “Legionnaires’”—the disease, the bacterium and methodology. Atlanta: Center for Disease Control.Google Scholar
  16. 16.
    Thomason, B. M., Chandler, F. W., Hollis, D. G. 1979. Flagella on Legionnaires’ disease bacteria: An interim report. Annals of Internal Medicine91:224–226.PubMedGoogle Scholar
  17. 17.
    Thomason, B. M., Harris, P. P., Hicklin, M. D., Blackmon, J. A., Moss, C. W., Matthews, F. 1979. Detection of aLegionella-like bacterium related to WIGA in a fatal case of pneumonia. Annals of Internal Medicine91:673–676.PubMedGoogle Scholar
  18. 18.
    Thornsberry, C., Kirven, L. A. 1978. β-Lactamase of the Legionnaires’ bacterium. Current Microbiology1:51–54.CrossRefGoogle Scholar
  19. 19.
    Weaver, R. E., Feeley, J. C. 1979. Cultural and biochemical characterization of the Legionnaires’ disease bacterium, pp. 19–25. In: Jones, G. L., Hébert, G. A. (eds.) “Legionnaires’”—the disease, the bacterium and methodology. Atlanta: Center for Disease Control.Google Scholar
  20. 20.
    Wilkinson, H. W., Farshy, C. E., Fikes, B. J., Cruce, D. D., Yealy, P. P. 1979. Measure of immunoglobulin G-, M-, and A-specific titers againstLegionella pneumophila and inhibition of titers against nonspecific, Gram-negative bacterial antigens in the indirect immunofluorescence test for legionellosis. Journal of Clinical Microbiology10:685–689.PubMedGoogle Scholar
  21. 21.
    Wilkinson, H. W., Fikes, B. J. 1980. Slide agglutination test for serogroupingLegionella pneumophila and atypicalLegionella-like organisms. Journal of Clinical Microbiology.11:99–101.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 1980

Authors and Affiliations

  • Don J. Brenner
    • 1
  • Arnold G. Steigerwalt
    • 1
  • G. W. Gorman
    • 2
  • Robert E. Weaver
    • 1
  • James C. Feeley
    • 2
  • L. G. Cordes
    • 2
  • Hazel W. Wilkinson
    • 1
  • Charlotte Patton
    • 2
  • Berenice M. Thomason
    • 1
  • Karen R. Lewallen Sasseville
    • 1
  1. 1.Bacteriology Division Center for Disease Control Public Health ServiceU. S. Department of Health and Human ServicesGeorgiaAtlantaUSA
  2. 2.Bacterial Diseases Division, Center for Disease Control, Public Health ServiceU. S. Department of Health and Human ServicesAtlantaUSA

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