Current Microbiology

, Volume 1, Issue 4, pp 209–211 | Cite as

Glucose transport in a glucosephosphate isomeraseless mutant ofSaccharomyces cerevisiae

  • Javier Perea
  • Carlos Gancedo


A glucosephosphate isomeraseless mutant ofSaccharomyces cerevisiae was able to accumulate glucose-6-phosphate when incubated with glucose. Transport ofd-xylose was measured in the glucose-6-phosphate charged cells and compared with untreated cells. Even at an intracellular concentration of 15 mM, inhibition of the transport did not surpass 20%. It is concluded that glucose-6-phosphate is not the regulatory metabolite of hexose transport in yeast.


Fermentation Xylose Fructose Glucose Transport Hexose Transport 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Azam, F., Kotyk, A. 1969. Glucose-6-phosphate as regulator of monosaccharide transport in baker’s yeast. FEBS Letters2:333–335.PubMedCrossRefGoogle Scholar
  2. 2.
    Bañuelos, M., Gancedo, C., Gancedo, J. M. 1977. Activation by phosphate of yeast phosphofructokinase. Journal of Biological Chemistry252:6394–6398.PubMedGoogle Scholar
  3. 3.
    Clifton, D., Weintock, S. B., Fraenkel D. G. 1978. Glycolysis mutants inS. cerevisiae. Genetics88: 1–11.PubMedGoogle Scholar
  4. 4.
    Gancedo, J. M., Gancedo, C. 1973. Concentration of intermediary metabolites in yeast. Biochimie55:205–211.PubMedGoogle Scholar
  5. 5.
    Heredia, C. F., Sols, A., De la Fuente, G. Specificity of the constitutive hexose transport in yeast. European Journal of Biochemistry,5:321–329.Google Scholar
  6. 6.
    Maitra, P. K. 1971. Glucose and fructose metabolism in a phosphoglucoisomeraselles mutant ofSaccharomyces cerevisiae. Journal of Bacteriology107:759–769.PubMedGoogle Scholar
  7. 7.
    Sáez, M. J., Lagunas, R. 1976. Determination of intermediary metabolites in yeast. Critical examination of the effect of sampling conditions and recommendations for obtaining true levels. Molecular and Cellular Biochemistry13:73–78.PubMedCrossRefGoogle Scholar
  8. 8.
    Serrano, R., De la Fuente, G. 1974. Regulatory properties of the constitutive hexose transport inS. cerevisiae. Molecular and Cellular Biochemistry5:161–171.PubMedCrossRefGoogle Scholar
  9. 9.
    Sols, A. 1976. The Pasteur effect in the allosteric era, pp. 199–206. In: Kornberg, A. et al. (eds.) Reflections in Biochemistry, Oxford: Pergamon Press.Google Scholar
  10. 10.
    Sprague, G. I. 1977. Isolation and characterization of aSaccharomyces cerevisiae mutant deficient in pyruvate kinase activity. Journal of Bacteriology130: 232–241.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 1978

Authors and Affiliations

  • Javier Perea
    • 1
  • Carlos Gancedo
    • 1
  1. 1.Instituto de Enzimologia del C. S. I. C.Facultad de Medicina de la Universidad AutónomaMadrid-34Spain

Personalised recommendations