Induction of aryl hydrocarbon hydroxylase inNeurospora crassa by benzo (α) pyrene
- 29 Downloads
WhenNeurospora crassa is grown on a minimal medium with sucrose as the carbon source, aryl hydrocarbon [benzo(α)pyrene] hydroxylase is induced in the presence of low concentrations of benzo(α)pyrene. Benzo(α)pyrene, a potent precarcinogen, is taken up readily by the growing mycelium and is metabolized by the intracellular enzymes to yield hydroxylated derivatives. Fractionation of the products by high pressure liquid chromatography following extraction in organic solvents revealed the presence of one major product. The purified major product was identified as 3-hydroxybenzo(α)pyrene by mass spectral analysis and by comparison of fluorescence emission and ultraviolet absorption spectra with authentic samples.
Unable to display preview. Download preview PDF.
- 12.Lu, A. Y. H., Lewin, W., West, S. B., Jacobson, M., Ryan, D., Kutzman, R., Conney, A. H. 1973. Reconstituted liver microsomal crosomal enzyme system that hydroxylates drugs, other foreign compounds, and endogenous substrates. VI. Different substrate specificities of the cytochrome P-450 fractions from control and phenobarbital-treated rats. Journal of Biological Chemistry248:456–460.PubMedGoogle Scholar
- 14.Miller, J. A., Miller, E. C. 1977. Ultimate chemical carcinogens as reactive mutagenic electrophiles, pp. 605–627. In: Hiatt, H. H., Watson, J. D., Winsten, J. A. (eds.), Origins of human cancer. Cold Spring Harbor: Cold Spring Harbor Laboratory.Google Scholar
- 15.Nebert, D. W. 1978. Genetic differences in microsomal electron transport: The Ah locus, pp. 226–240. In: Fleischer, S., Packer, L. (eds.), Methods in enzymology, vol. 52. New York: Academic Press.Google Scholar
- 17.Sato, R., Omura T. 1978. Cytochrome P-450. Tokyo, New York: Kadansha and Academic Press.Google Scholar
- 19.Vogel, H. J. 1956. A convenient growth medium forNeurospora crassa (N medium). Microbial Genetics Bulletin13:42–43.Google Scholar