Comparison of four in vitro assays for specific IgE detection

  • Paola Boccagni
  • Flavio Favari
  • Giovanna Zanoni
  • Angela Pezzini
  • Giuseppe Tridente
Originals

Summary

Three immunoenzymatic techniques for specific IgE detection (Pharmacia CAP System, Kallestad Allercoat System, Neo Abellò Hamlet-IgE) and the classical Phadebas RAST were compared using 34 sera from patients with a clinical diagnosis of allergic disease and 19 sera from healthy non-atopic controls. IgE antibodies to 9 aeroallergens and 6 food antigens were assessed and 399 tests were run with each method. All techniques showed a high specificity (92%–100%) and satisfactory efficiency (82%–98%), while the sensitivity for RAST, CAP, Allercoat and Hamlet was 89%, 91%, 83% and 53%, respectively, with the lowest values for food allergens. There was a good overall correlation of the four techniques, except when the Hamlet method was compared with the other methods for food-specific IgE detection (correlation coefficient <0.3). These data indicate that CAP, Allercoat and RAST are satisfactory techniques for specific IgE determination, either for inhalants or for food allergens; CAP, however, offers the highest sensitivity without loss of specificity.

Key words

Allergy diagnosis Comparison of immunoenzymatic assays RAST Specific IgE 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Paola Boccagni
    • 1
  • Flavio Favari
    • 1
  • Giovanna Zanoni
    • 1
  • Angela Pezzini
    • 1
  • Giuseppe Tridente
    • 1
  1. 1.Institute of Immunology and Infectious DiseasesUniversity of VeronaVeronaItaly
  2. 2.Institute of Immunology and Infectious DiseasesPoliclinico Borgo RomaVeronaItaly

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