Pflügers Archiv

, Volume 415, Issue 5, pp 645–647 | Cite as

pH modulates conducting and gating behaviour of single calcium release channels

  • Eric Rousseau
  • Janet Pinkos
Short Communication Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology

Abstract

Intracellular pH changes affect excitation-contraction coupling in skeletal, and cardiac muscles. However the proton implication in modulating the sarcoplasmic reticulum Ca2+ release channel activity has never been visualized at single channel level. A large conducting Ca2+ release pathway has previously been characterized after incorporation of skeletal and cardiac sarcoplasmic reticulum vesicles into planar lipid bilayers. This channel has been activated by micromolar and millimolar concentrations of Ca2+ and ATP, respectively. The pH was independently varied on each side of the channels. Acidification of the cis-chamber (7.4 to 6.6) induced a modification of the gating behaviour, resulting in a decrease of the open probability. This effect was completely reversible. On the other hand, acidification of the trans-chamber (7.4 to 6.8) induced a reduction of the unitary conductance of the sarcoplasmic reticulum Ca2+ release channel.

Key words

Ca2+ release sarcoplasmic reticulum planar lipid bilayer membrane reconstitution pH 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. DeHemptinne A, Marrannes R, Vanhell B (1987) Surface pH and the control of intracellular pH in cardiac and skeletal muscle. Can J Physiol Pharmacol 65: 970–977Google Scholar
  2. Donghee K, Clapham DE (1989) Potassium channels in cardiac cells activated by arachidonic acid and phospholipids. Science, 244: 1174–1176CrossRefGoogle Scholar
  3. Fabiato A (1981) Myoplasmic free calcium concentration reached during the twich of an intact isolated cardiac cell. J Gen Physiol 78: 457–497PubMedCrossRefGoogle Scholar
  4. Lai AF, Erickson HP, Rousseau E, Liu QY, Meissner G (1988) Purification and reconstitution of the calcium release channel from skeletal muscle. Nature 331: 313–319CrossRefGoogle Scholar
  5. Mainwood G, Renaud JM, Masson MJ (1987) The pH dependence of the contractile response of fatigued skeletal muscle. Can J Physiol Pharmacol 65: 648–658PubMedGoogle Scholar
  6. Meissner G, Henderson JS (1987) Rapid calcium release from cardiac sarcoplasmic reticulum vesicles is dependent on Ca2+ and is modulated by Mg2+, adenine nucleotide and calmodulin. J Biol Chem 262: 3065–3073PubMedGoogle Scholar
  7. Michalak M (1988) Identification of the Ca2+ release activity and ryanodine receptor in sarcoplasmic-reticulum membranes during cardiac myogenesis. Biochem J 253: 631–636PubMedGoogle Scholar
  8. Prod'hom B, Pietrobon D, Hess P (1987) Direct measurement of proton transfer rates to a group controlling the dihydropyridine-sensitive Ca2+ channel. Nature 329: 243–246PubMedCrossRefGoogle Scholar
  9. Rousseau E, Ladine J, Meissner G (1988) Activation of the Ca2+ release channel of skeletal muscle sarcoplasmic reticulum by caffeine and related compounds. Arch Biochem Biophys 267: 75–86PubMedCrossRefGoogle Scholar
  10. Rousseau E, Smith JS, Henderson JS, Meissner G (1986) Single channel and45Ca2+ flux measurements of the cardiac sarcoplasmic reticulum calcium channel. Biophys J 50: 1009–1014PubMedCrossRefGoogle Scholar
  11. Su JY, Hasselbach W (1984) Caffeine-induced calcium release from isolated sarcoplasmic reticulum of rabbit skeletal muscle. Eur J Physiol 400: 14–21CrossRefGoogle Scholar
  12. Takeshima H, Seiichiro N, Takeshi M et al (1989) Primary structure and expression from complementary DNA of skeletal muscle ryanodine receptor. Nature 339: 439–445PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Eric Rousseau
    • 1
  • Janet Pinkos
    • 1
  1. 1.Department of Physiology and Biophysics, Faculty of MedicineUniversity of SherbrookeSherbrookeCanada

Personalised recommendations