Acta diabetologia latina

, Volume 14, Issue 5–6, pp 263–272 | Cite as

The influence of pentoxyfylline [1-(5-oxohexyl-) 3,7-dimethylxanthine] (BL 191) on the insulin secretion induced by glibenclamide and by arginine/glucose in the perfused pancreas

  • Juan C. Basabe
  • Daniel P. Udrisar
  • Carlos F. Knopf
  • Nestor Aparicio


Pharmacodynamic characteristics of pentoxyfylline (BL 191) related to insulin secretion by the isolated perfused rat pancreas are studied.

The results obtained show that: 1) BL 191 (5 mM) is capable of stimulating insulin secretion, even in the presence of another stimulator; 2) BL 191 increases both phases of the secretion produced by constant arginine 20 mM/glucose 5 mM perfusion; 3) BL 191 significantly increases and turns biphasic the monophasic insulin secretion pattern produced by 1 µg/ml glibenclamide; 4) the effects mentioned in points 2) and 3) are inhibited if the phosphodiesterase activator imidazole (300 mg/100 ml) is present in the perfusion medium; 5) the phosphodiesterase inhibitor theophylline has the same effects as BL 191, except for its inability to stimulate insulin release in the absence of another stimulator; 6) somatostatin (100 ng/ml) significantly inhibits insulin secretion produced by arginine/glucose or glibenclamide, as well as by arginine, glucose plus theophylline or BL 191, and by glibenclamide plus theophylline or BL 191; in both cases the inhibitory effect of somatostatin is reduced by the presence of BL 191 or theophylline.


Insulin Secretion Theophylline Glibenclamide Insulin Release Inhibit Insulin Secretion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1).
    Alberti K. G. M. M., Christensen S. E., Iversen J., Seyer-Hansen K., Christensen N. J., Hansen A. P., Lundbaek K., Ørskov H.: Inhibition of Insulin Secretion by Somatostatin — Lancet2, 1299, 1973.Google Scholar
  2. 2).
    Basabe J. C., Cresto J. C., Aparicio N., Schally A. V.: Studies on the Mode of Action of Somatostatin on Insulin Secretion — Endocrinology, 1977 (in press).Google Scholar
  3. 3).
    Basabe J. C., Lopez N. L., Viktora J. K., Wolff F. W.: Studies of Insulin Secretion in the Perfused Rat Pancreas. Effect of Diazoxide and A025 — Diabetes19, 271, 1970.PubMedGoogle Scholar
  4. 4).
    Basabe J. C., Lopez N. L., Viktora J. K., Wolff F. W.: Insulin Secretion Studied in the Perfused Rat Pancreas. II. Effect of Glucose, Glucagon, 3′, 5′ Adenosine Monophosphate, Theophylline, Imidazole and Phenoxybenzamine; their Interaction with Diazoxide — Diabetes20, 457, 1971.PubMedGoogle Scholar
  5. 5).
    Boksay I., Bollmann V.: The Effect of 3,7-Dimethyl-1(5-Oxohexyl)-Xanthine on the Adrenergic Receptors and on the Activity of Isoprenaline — Arch. int. Pharmacdoyn.194, 174, 1974.Google Scholar
  6. 6).
    Cerasi E., Luft R.: The Effect of an Adenosine-3′, 5′-Monophosphate Diesterase Inhibitor (Aminophylline) on the Insulin Response to Glucose Infusion in Prediabetic and Diabetic Subjects — Hormone metab. Res.1, 162, 1969.Google Scholar
  7. 7).
    Charles M. A., Fanska R., Schmid F. D., Grodsky G. M.: Adenosine 3′, 5′-Monophosphate in Pancreatic Islets: Glucose Induced Insulin Release — Science179, 569, 1973.PubMedCrossRefGoogle Scholar
  8. 8).
    Cresto J. C., Dujovne I. L., Castellani P. I., Mitta E. A., De Majo S. F., Foglia V. G.: Insulin Radioimmunoassay by the Charcoal-Dextran Technique — Diabetologia8, 292, 1972.PubMedCrossRefGoogle Scholar
  9. 9).
    Curry D., Bennett L. L., Grodsky G. M.: Dynamics of Insulin Secretion by the Perfused Rat Pancreas — Endocrinology83, 72, 1968.Google Scholar
  10. 10).
    Dollinger H. C., Raptis S., Grebe B., Goebell H., Müller R., Hinze H., Pfeiffer E. F.: The Influence of 1-(5-Oxohexyl-)3,7-Dimethylxantine (BL 191) on the Endocrine and Exocrine Pancreatic Function in Man during and following Secretion and Cholecystokinin-Pancreozymin Stimulation — Res. exp. Med.161, 327, 1973.CrossRefGoogle Scholar
  11. 11).
    Efendić S., Luft R.: Studies on the Inhibitory Effect of Somatostatin on Glucose Induced Insulin Release in the Isolated Perfused Rat Pancreas — Acta endocr. (Kbh.)78, 510, 1975.Google Scholar
  12. 12).
    Fujimoto W. Y., Ensinck J. W.: Somatostatin Inhibition of Insulin and Glucagon Secretion in Rat Islet Culture: Reversal by Ionophore A 23187 — Endocrinology98, 259, 1976.PubMedCrossRefGoogle Scholar
  13. 13).
    Gerich J. E., Lorenzi M., Schneider V., Forsham P. H.: Effect of Somatostatin on Plasma Glucose and Insulin Responses to Glucagon and Tolbutamide in Man — J. clin. Endocr.39, 1057, 1974.PubMedCrossRefGoogle Scholar
  14. 14).
    Herbert V., Lan K., Gottlieb S., Bleicher S.: Coated Charcoal Radioimmunoassay of Insulin — J. clin. Endocr.25, 1375, 1965.PubMedGoogle Scholar
  15. 15).
    Malaisse W., Malaisse-Lagae F., King S.: Effect of Neutral Red and Imidazole upon Insulin Secretion — Diabetologia4, 370, 1968.PubMedCrossRefGoogle Scholar
  16. 16).
    Malaisse W., Malaisse-Lagae F., Mayhew D.: A Possible Role for the Adenylcyclase System in Insulin Secretion — J. clin. Invest.46, 1724, 1967.PubMedGoogle Scholar
  17. 17).
    Montague W., Cock J. R.: The Role of Adenosine 3′, 5′-Cyclic Monophosphate in the Regulation of Insulin Release by Isolated Rat Islets of Langerhans — Biochem. J.122, 115, 1971.PubMedGoogle Scholar
  18. 18).
    Penhos J. C., Wu C., Basabe J. C., Lopez N. L., Wolff F.: A Rat Pancreas Small Gut Preparation for the Study of Intestinal Factor(s) and Insulin Release — Diabetes18, 733, 1969.PubMedGoogle Scholar
  19. 19).
    Porte D. Jr., Pupo A.: Insulin Release to Glucose: Evidence for a Two Pool System in Man — J. clin. Invest.48, 2309, 1969.PubMedCrossRefGoogle Scholar
  20. 20).
    Sieradzki J., Schatz H., Nierle Ch., Pfeiffer E. F.: Somatostatin-Induced Inhibition of Insulin Secretion from Isolated Islets of Rat Pancreas in Presence of Glucagon — Hormone metab. Res.7, 284, 1975.CrossRefGoogle Scholar
  21. 21).
    Turtle J., Kipnis D.: Stimulation of Insulin Secretion by Theophylline — Nature (Lond.)213, 727, 1967.CrossRefGoogle Scholar

Copyright information

© Casa Editrice «IL PONTE» 1977

Authors and Affiliations

  • Juan C. Basabe
    • 1
    • 3
  • Daniel P. Udrisar
    • 3
  • Carlos F. Knopf
    • 3
  • Nestor Aparicio
    • 2
    • 3
  1. 1.the Carrera del Investigador CientíficoConsejo Nacional de Investigaciones CientíficasArgentina
  2. 2.Grupo de Estudios en Fertilidad y Endocrinología de Rosario (G.E.F.E.R.)RosarioArgentina
  3. 3.Fundación Laboratorios de Investigaciones Pediátricas (F.L.I.P.)Hospital de Pediatría ‘Dr. P. de Elizalde’Buenos AiresArgentina

Personalised recommendations