Enhanced proliferation of coronary endothelial cells in response to growth factors is synergized by hemodialysate compounds in vitro
- 37 Downloads
Using capillary endothelial cells from the coronary system of guinea pig heart, we have devised an in vitro assay suitable for measuring proliferative and growth-promoting effects mediated by drugs and growth factors. In this assay, hemodialysate and fractions isolated from it—consisting exclusively of low-molecular-weight compounds from calf blood—stimulate the proliferation of coronary endothelial cells. This effect is not a trivial nutritive one. Furthermore, in respect to the stimulation of proliferation, hemodialysate synergizes with epidermal growth factor, basic fibroblast growth factor, and endothelial cell growth factor, but not with insulin, which shows no proliferative effect in our system. From data obtained by analysis of hemodialysate fractions, it is deduced that the active compounds are strongly negatively charged oligosaccharides with a molecular weight of apparently 3000 Dalton. The synergistic effect of hemodialysate compounds on proliferation of endothelial cells as well as their previously demonstrated insulin-like activity is believed to explain the therapeutic efficacy of hemodialysate in cases of impaired wound healing and occlusive diseases.
Key wordsEndothelial cells Growth factors Insulin Angiogenesis Wound healing
Unable to display preview. Download preview PDF.
- 8.Carson MP, Saenz de Tejada I, Goldstein I, haudenschild CC (1989) Culture of human corpus cavernosum endothelium. In Vitro 25:248–254Google Scholar
- 12.Dichek D, Quertermous T (1989) Variability in messenger RNA levels in human umbilical vein endothelial cells of different lineage and time in culture. In Vitro 25:289–292Google Scholar
- 13.Folkman J (1984) Angiogenesis. In: Jaffe EA (ed) Biology of endothelial cells (Chapter 42). Martinus Nijhoff, Boston, pp 413–428Google Scholar
- 15.Gospodarowicz D, Savion N, Giguere L (1982) The control of proliferation and differentiation of endothelial cells. In: Nossel HL, Vogel HJ (eds) Pathobiology of the endothelial cell. Academic Press, New York, pp. 19–61Google Scholar
- 21.Hoshi H, Kan M, Chen J-K, McKeehan WL (1988) Comparative endocrinology-paracrinology-autocrinology of human adult large vessel endothelial and smooth muscle cells. In Vitro 24:309–320Google Scholar
- 22.Ingber DE, Madri JA, Folkman J (1987) Endothelial growth factors and extracellular matrix regulate DNA synthesis through modulation of cell and nuclear expansion. In Vitro 23:387–394Google Scholar
- 26.Mansson P-E, Malark M, Sawada H, Kan M, McKeehan WL (1990) Heparin-binding (fibroblast) growth factor type one and two genes are co-expressed in proliferating normal human vascular endothelial and smooth muscle cells in culture. In Vitro 26:209–212Google Scholar
- 35.Rupnick MA, Carey A, Williams SK (1988) Phenotypic diversity in cultured cerebral microvascular endothelial cells. In Vitro 24:435–444Google Scholar
- 37.Smahel J (1982) Effect of a protein-free hemodialysate on the recovery of blood circulation in an ischaemic skin lesion. Br J Exp Pathol 63:117–183Google Scholar
- 38.Straczek J, Heulin MH, Sarem F, Lasbennes A, Artur M, Geschier C, Stoltz JF, Belleville F, Nabet P, Maquart FX, Gillery P, Borel J, Herman A, Lebeurre MD, DeLavergne E, Genetet N (1983) Effect of partially purified preparation of human somatomedin A in the three cultured cell systems. In: Fischer G, Wieser RJ (eds) Hormonally defined media. Springer, Berlin Heidelberg New York, pp 127–130Google Scholar