Archives of Microbiology

, Volume 164, Issue 1, pp 36–42 | Cite as

The role of an NAD-independent lactate dehydrogenase and acetate in the utilization of lactate byClostridium acetobutylicum strain P262

  • Francisco Diez-Gonzalez
  • James B. Russell
  • Jean B. Hunter
Original Paper

Abstract

Clostridium acetobutylicum strain P262 utilized lactate at a rapid rate [600 nmol min−1 (mg protein)−1], but lactate could not serve as the sole energy source. When acetate was provided as a co-substrate, the growth rate was 0.05h−1. Butyrate, carbon dioxide and hydrogen were the end products of lactate and acetate utilization, and the stoichiometry was 1 lactate + 0.4 acetate →0.7 butyrate + 0.6H2 + 1CO2. Lactate-grown cells had twofold lower hydrogenase than glucose-grown cells, and the lactate-grown cells used acetate as an alternative electron acceptor. The cells had a poor affinity for lactate (Ks=1.1 mM), and there was no evidence for active transport. Lactate utilization was catabolyzed by an inducible NAD-independent lactate dehydrogenase (iLDH) that had a pH optimum of 7.5. The iLDH was fivefold more active withd-lactate thanl-lactate, and theKm ford-lactate was 3.2 mM. Lactate-grown cells had little butyraldehyde dehydrogenase activity, and this defect did not allow the conversion of lactate to butanol.

Key words

Clostridium acetobutylicum Lactate utilization Acetate utilization Acetone-butanol fermentation Lactate dehydrogenase 

Abbreviations

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PMS

phenazine methosulfate

nLDH

NAD-dependent lactate dehydrogenase

iLDH

NAD-independent lactate dehydrogenase

Fru-1,6-P2

fructose-1,6-bisphosphate

DTT

dithiothreitol

ΔΨ

membrane potential

TPP+

tetraphenylphosphonium bromide

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Francisco Diez-Gonzalez
    • 2
  • James B. Russell
    • 1
  • Jean B. Hunter
    • 3
  1. 1.Agricultural Research Service, USDA and Section of Microbiology, Wing HallCornell UniversityIthacaUSA
  2. 2.Department of Food ScienceCornell UniversityIthacaUSA
  3. 3.Department of Agricultural and Biological EngineeringCornell UniversityIthacaUSA

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