Antonie van Leeuwenhoek

, Volume 41, Issue 1, pp 249–256 | Cite as

Regulation of citrate synthase activity ofSaccharomyces cerevisiae

  • J. S. Coleman
  • J. K. Bhattacharjee
Article

Abstract

Citrate synthase activity ofSaccharomyces cerevisiae was determined by a radioactive assay procedure and the reaction product,14C-citric acid, was identified by chromatographic techniques. ATP, d-ATP, GTP and NADPH were most inhibitory to the citrate synthasein vitro. The activity was inhibited to a lesser extent by ADP, UTP, and NADP whereas, AMP and CTP were much less inhibitory. NADH, like NAD, glutamic acid, glutamine, arginine, ornithine, proline, aspartic acid and α-ketoglutarate exhibited no inhibition. These results have been discussed in the light of the role of citrate synthase for the energy metabolism and glutamic acid biosynthesis.

Keywords

NADH Adenosine Triphosphate Tricarboxylic Acid Cycle Ketoglutarate Tricarboxylic Acid Cycle Intermediate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© H. Veenman & Zonen B.V. Publishers 1975

Authors and Affiliations

  • J. S. Coleman
    • 1
  • J. K. Bhattacharjee
    • 1
  1. 1.Department of MicrobiologyMiami UniversityOxfordUSA

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