Calcified Tissue International

, Volume 37, Issue 5, pp 453–460

Human bone cellsin vitro

  • Pamela Gehron Robey
  • John D. Termine
Clinical Investigations

DOI: 10.1007/BF02557826

Cite this article as:
Robey, P.G. & Termine, J.D. Calcif Tissue Int (1985) 37: 453. doi:10.1007/BF02557826

Summary

Human bone cell cultures were established by maintaining collagenase-treated, bone fragments in low Ca++ medium. The resulting cell cultures exhibited a high level of alkaline phosphatase activity and produced a significant increase in intracellular cAMP when exposed to the 1–34 fragment of human parathyroid hormone. With continued culture, the cells formed a thick, extracellular matrix that mineralized when cultures were provided daily with normal levels of calcium, fresh ascorbic acid (50 μg/ml) and 10 mM β-glycerol phosphate. Biosynthetically, these cells produced type I collagen (without any type III collagen), and the bone-specific protein, osteonectin. In addition, the cells produced sulfated macromolecules electrophoretically identical to those positively identified as the bone proteoglycan in parallel cultures of fetal bovine bone cells. This technique provides a useful system for the study of osteoblast metabolismin vitro

Key words

Osteoblasts Extracellular matrix Cell culture Bone-specific proteins 

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Pamela Gehron Robey
    • 1
  • John D. Termine
    • 1
  1. 1.Mineralized Tissue Research Branch, National Institute of Dental ResearchNational Institutes of HealthBethesdaUSA

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