Calcified Tissue International

, Volume 37, Issue 1, pp 82–90 | Cite as

1α,25-Dihydroxyvitamin D3 receptors and their action in embryonic chick chondrocytes

  • Satoshi Suda
  • Naoyuki Takahashi
  • Toshimasa Shinki
  • Noboru Horiuchi
  • Akira Yamaguchi
  • Shusaku Yoshiki
  • Shoji Enomoto
  • Tatsuo Suda
Laboratory Investigations

Summary

The role of vitamin D in the maturation of epiphyseal chondrocytes was investigated in the developing chick embryo. Cartilage tissues were divided into two parts: resting cartilage and growth cartilage. A cytosol component to which 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3) is specifically bound first appeared in the growth cartilage on day 15, rapidly increased, and attained a maximum on day 19. The calcium content of the growth cartilage also began to increase on day 15 and continued to increase in parallel with the 1α,25(OH)2D3 receptor levels. Glycosaminoglycan (GAG) synthesis by the growth cartilage cells increased from day 11–17 and rapidly declined thereafter reciprocally with the increase in calcium and receptor levels. In the resting cartilage, no cytosol receptor for 1α,25(OH)2D3 was detected up to hatching time. The calcium content and GAG synthesis in the resting cartilage were very low and did not change appreciably throughout development. No receptor-like macromolecule for 24R,25-dihydroxyvitamin D3 (24R,25(OH)2D3) was recognized in either the resting or growth cartilage. 1α,25(OH)2D3 added to the culture of chondrocytes from the epiphyseal growth cartilage inhibited GAG synthesis and stimulated its release from the cell layer into the medium in a dose-dependent manner. Thesein vitro effects of 1α,25(OH)2D3 were not observed in chondrocytes obtained from 13-day-old growth cartilage and 19-day-old resting cartilage. 25-Hydroxyvitamin D3 and 24R,25(OH)2D3 had no effect on chondrocytes in any of the preparations. These results suggest that 1α,25(OH)2D3 is directly involved in the maturation of chondrocytes and possibly in the calcification of growth cartilage.

Key words

Embryonic chick chondrocytes 1α,25-Dihydroxyvitamin D3 1α,25-Dihydroxyvitamin D3 receptor Glycosaminoglycan Calcification of cartilage 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Satoshi Suda
    • 1
  • Naoyuki Takahashi
    • 2
  • Toshimasa Shinki
    • 2
  • Noboru Horiuchi
    • 2
  • Akira Yamaguchi
    • 3
  • Shusaku Yoshiki
    • 3
  • Shoji Enomoto
    • 1
  • Tatsuo Suda
    • 2
  1. 1.Second Department of Oral Surgery, Faculty of DentistryTokyo Medical and Dental UniversityTokyo
  2. 2.Department of Biochemistry, School of DentistryShowa UniversityTokyoJapan
  3. 3.Department of Oral Pathology, School of DentistryShowa UniversityTokyoJapan

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