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Calcified Tissue International

, Volume 37, Issue 1, pp 51–56 | Cite as

Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone and calcitonin

  • S. M. Forrest
  • K. W. Ng
  • D. M. Findlay
  • V. P. Michelangeli
  • S. A. Livesey
  • N. C. Partridge
  • J. D. Zajac
  • T. J. Martin
Laboratory Investigations

Summary

The clonal cell line UMR 106, which was originally derived from a rat transplantable osteogenic sarcoma with an osteoblastic phenotype, was subcloned after the emergence of a calcitonin-responsive adenylate cyclase was noted in late passages. Detailed studies on the stimulation of adenylate cyclase and activation profile of the cyclic AMP-dependent protein kinase isoenzymes in response to parathyroid hormone (PTH) and salmon calcitonin (SCT) were conducted on two subclones (UMR 106-01 and UMR 106-06). Both subclones responded in an identical manner to PTH, which stimulated adenylate cyclase and activated both isoenzyme I and isoenzyme II of cyclic AMP-dependent protein kinase. In contrast, only UMR 106-06 cells responded to calcitonin. At 3×10−8M SCT, there was a sevenfold stimulation of adenylate cyclase, 84% activation of isoenzyme I, and 44% activation of isoenzyme II. The activation profiles of the isoenzymes to PTH and SCT in UMR 106-06 were similar. Furthermore, their response to SCT correlates with the presence of specific, saturable binding of125I-labeled SCT. Binding parameters indicate apparent Kd=0.8 nM and 6,000 receptors/cell. These data point to a significant phenotypic change having taken place in this clonal cell line with prolonged maintenance in culture, with the emergence of a calcitonin receptor linked to adenylate cyclase and protein kinase activation.

Key words

Osteoblast Parathyroid hormone Calcitonin Cyclic AMP Differentiation 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • S. M. Forrest
    • 1
  • K. W. Ng
    • 1
  • D. M. Findlay
    • 2
  • V. P. Michelangeli
    • 1
  • S. A. Livesey
    • 1
  • N. C. Partridge
    • 1
  • J. D. Zajac
    • 1
  • T. J. Martin
    • 1
  1. 1.Department of Medicine, Repatriation General HospitalUniversity of MelbourneHeidelbergAustralia
  2. 2.Department of Clinical PharmacologyAustin HospitalHeidelbergAustralia

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