Expression of bone sialoprotein (BSP) in developing human tissues
- 294 Downloads
Bone sialoprotein (BSP) and its messenger RNA were localized in developing human skeletal and nonskeletal tissues by means of immunohistochemistry andin situ hybridization. Both protein and mRNA were found in mature, bone-forming cells but not in their immature precursors. In addition, osteoclasts displayed positive immunostaining and high densities of autoradiographic grains byin situ hybridization experiments. BSP was expressed in fetal epiphyseal cartilage cells, particularly in hypertrophic chondrocytes of growth plates. Though neither the protein nor the mRNA were identified in a variety of other connective and nonconnective tissues, an unexpected finding was the expression of BSP in the trophoblast cells of placenta. These findings show that BSP is primarily an osteoblast-derived component of the bone matrix expressed at late stages of differentiation. We have also found that osteoclasts produce BSP, possibly as a mediator of cell attachment to bone.
Key wordsBone sialoprotein Developing bone Noncollagenous proteins In situ hybridization Osteoblast Osteoclast Cell adhesion
Unable to display preview. Download preview PDF.
- 1.Herring GM (1972) The organic matrix of bone. In: Bourne GH (ed) The biochemistry and physiology of bone, vol 1. Academic Press, New York, pp 127–189Google Scholar
- 3.Fisher LW, Termine JD, Young MF (in press) Human bone sialoprotein: deduced protein sequence and chromosomal localization. J Biol ChemGoogle Scholar
- 11.Mintz KP, Midura RJ, Gehron Robey P, Termine JD, Fisher LW (1990) Attachment properties of non-denatured rat bone sialoprotein. J Bone Miner Res 5:S232Google Scholar
- 20.Bianco P, Fisher LW, Young MF, Kopp JB, Termine JD, Gehron Robey P (1990) The use of synthetic peptide antibodies and in situ hybridization for investigating expression and localization of small proteoglycans of developing bone (biglycan and decorin). In: Cohn DV, Glorieux FH, Martin TJ (eds) Proceedings ASBMR/ICCRH. Elsevier, Amsterdam, pp 201–206Google Scholar