Calcified Tissue International

, Volume 49, Issue 6, pp 383–388 | Cite as

Resolution-enhanced fourier transform infrared spectroscopy study of the environment of phosphate ion in the early deposits of a solid phase of calcium phosphate in bone and enamel and their evolution with age: 2. Investigations in thev 3 PO4 domain

  • C. Rey
  • M. Shimizu
  • B. Collins
  • M. J. Glimcher
Laboratory Investigations

Summary

Resolution-enhanced Fourier Transform Infrared (FTIR) spectra of early mineral deposits in enamel and bone show bands at 1020, 1100, 1110, 1125, and 1145 cm−1 in thev 3PO4 domain which do not belong to well crystallized stoichiometric hydroxyapatite. Bands at 1020 and 1100 cm−1 have been shown to occur in nonstoichiometric apatites containing HPO 4 2− ions. Though the bands at 1110 and 1125 cm−1 have not been found in any well crystallized apatite, they are present in newly precipitated apatite. These latter bands disappear progressively during maturation in biological as well as synthetic samples, and partial dissolution of synthetic apatites shows that they belong to species that exhibit an inhomogeneous distribution in the mineral, and that are the first to be solubilized. Comparison of the FTIR spectra of biological apatites with those of synthetic, nonapatitic-containing phosphate minerals shows that the presence of these bands does not arise from nonapatitic, well-defined phases; they are due to the local environment of phosphate ions which may possibly be loosely related or perhaps unrelated to the phosphate groups present in the well-crystallized nonapatitic calcium phosphates. Resolution-enhanced FTIR affords a very precise characterization of the mineral phases which may be very useful in characterizing pathological deposits of Ca−P mineral phases.

Key words

Infrared spectroscopy Bone Enamel Carbonate Phosphate 

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References

  1. 1.
    Rey C, Collins B, Goehl T, Shimizu M, Glimcher MJ (1990) Resolution-enhanced Fourier infrared spectroscopy study of the environment of phosphate ions in the early deposits of a solid phase of calcium phosphate in bone and enamel, and their evolution with age. I. Investigation in thev 4PO4 domain. Calcif Tissue Int 46:384–394PubMedCrossRefGoogle Scholar
  2. 2.
    Fowler BO (1974) Infrared studies of apatites. I. Vibrational assignments for calcium, strontium and barium hydroxyapatites utilizing isotopic substitution. Inor Chem 13:194–207CrossRefGoogle Scholar
  3. 3.
    Trombe JC (1972) Contribution a l'etude de la decomposition et de la reactivite de certaines apatites hydroxylees, carbonatees ou fluourees alcalino-terreuses. These d'Etat, Universite Paul Sabatier, ToulouseGoogle Scholar
  4. 4.
    Hannah RW, Swinehart JS (1974) Experiments in techniques of infrared spectroscopy. Perkin-Elmer, Norwalk, ConnGoogle Scholar
  5. 5.
    Rey C, Collins B, Goehl T, Glimcher MJ (1989) The carbonate environment in bone mineral. A resolution-enhanced Fourier transform infrared spectroscopy study. Calcif Tissue Int 45:157–164PubMedCrossRefGoogle Scholar
  6. 6.
    Labarthes JC, Bonel G, Montel G (1973) Sur la structure et les proprietes des apatites carbonatees de type B phosphocalciques. Ann Chim 8:289–301Google Scholar
  7. 7.
    Heughebaert JC (1977) Contribution a l'etude de l'evolution des orthophosphates amorphes en phosphates apatitiques. These d'Etat, Institut National Polytechnique de ToulouseGoogle Scholar
  8. 8.
    LeGeros RZ, Trautz OR, LeGeros JP, Klein E (1968) Carbonate substitution in the apatitic structure. Bull Soc Chim Fr (special issue):1712–1718Google Scholar
  9. 9.
    Meyer JL, Fowler BO (1982) Lattice defects in nonstoichiometric calcium hydroxyapatites. A chemical approach. Inorg Chem 21:3029–3035CrossRefGoogle Scholar
  10. 10.
    Berry EE (1967) The structure and composition of some calcium-deficient apatites. J Inorg Nucl Chem 29:317–327CrossRefGoogle Scholar
  11. 11.
    Fowler BO, Moreno EC, Brown WE (1966) Infrared spectra of hydroxyapatite, octacalcium phosphate and pyrolysed octacalcium phosphate. Arch Oral Biol 11:477–492PubMedCrossRefGoogle Scholar
  12. 12.
    Brown WE, Schroeder LW, Ferris JS (1979) Interlayering of crystalline octacalcium phosphate and hydroxyapatite. J Phys Chem 83:1835–1838CrossRefGoogle Scholar
  13. 13.
    Petrov I, Soptrajanov B, Fuson N, Lawson JR (1967) Infrared investigation of dicalcium phosphates. Spectrochimia Acta 23A:2637–2646CrossRefGoogle Scholar
  14. 14.
    Brown WE (1966) Crystal growth of bone mineral. Clin Orthop 44:205–220PubMedGoogle Scholar
  15. 15.
    Termine JD, Posner AS (1966) Amorphous/crystalline interrelationships in bone mineral. Calcif Tissue Res 1:8–23CrossRefGoogle Scholar
  16. 16.
    Francis MD, Webb NC (1971) Hydroxyapatite formation from hydrated calcium monohydrogen phosphate precursor. Calcif Tissue Res 6:335–342PubMedCrossRefGoogle Scholar
  17. 17.
    Young RA (1975) Some aspects of crystal structural modeling of biological apatites. Proc. of Physicochimie et Cristallographie des apatites d'interet biologiques. CNRS ParisGoogle Scholar
  18. 18.
    Legros R, Balmain N, Bonel G (1986) Structure and composition of the mineral phase of periosteal bone. J Chem Res Synop 1:8–9Google Scholar
  19. 19.
    Rey C, Beshah K, Griffin B, Glimcher MJ (in press) Structural studies of the mineral phase of calcifying cartilage. J Bone Min ResGoogle Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • C. Rey
    • 4
  • M. Shimizu
    • 2
  • B. Collins
    • 3
  • M. J. Glimcher
    • 1
  1. 1.Labratory for the Study of Skeletal Disorders and Rehabilitation, The Children's HospitalHarvard Medical SchoolBostonUSA
  2. 2.School of Dental MedicineTsurumi UniversityYokohamaJapan
  3. 3.Department of ChemistryNational Institute of Environmental Health ScienceResearch Triangle ParkUSA
  4. 4.Laboratoire de Physico-chimie des SolidesC.N.R.S. (UA 445)ToulouseFrance

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