Journal of the American Oil Chemists’ Society

, Volume 70, Issue 3, pp 225–228

Proton nuclear magnetic resonance rapid and structure-specific determination ofω-3 polyunsaturated fatty acids in fish lipids

  • Raffaele Sacchi
  • Isabel Medina
  • Santiago P. Aubourg
  • Francesco Addeo
  • Livio Paolillo


Based on proton nuclear magnetic resonance (1H-NMR) spectroscopy, a rapid and structure-specific method for the determination of ω-3 polyunsaturated fatty acids (PUFAs) in fish lipids is presented. The different chemical shift observed for the methyl resonance of ω-3 PUFAs (δ=0.95 ppm) with respect to the methyl resonance of all other fatty acids (δ=0.86 ppm) has provided the possibility of proposing a new and rapid method for the determination of ω-3 PUFA content. Twenty-four fish lipid samples (raw, cooked and canned albacore tuna) produced results that showed good agreement between1H-NMR analysis and gas chromatographic determination. Raw and cooked samples showed significantly higher levels of ω-3 PUFA than canned tuna.

Key words

1D-TOCSY CG 1H-NMR ω-3 PUFA determination fresh cooked and canned tuna fish oils Thunnus alalunga lipids 


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Copyright information

© The American Oil Chemists’ Society 1993

Authors and Affiliations

  • Raffaele Sacchi
    • 1
  • Isabel Medina
    • 1
  • Santiago P. Aubourg
    • 1
  • Francesco Addeo
    • 1
  • Livio Paolillo
    • 1
  1. 1.Istituto di Industrie AgrarieUniversità di Napoli Federico IIPorticiItaly
  2. 2.Instituto de Investigaciones Marinas del CSICVigoSpain
  3. 3.Dipartimento di ChimicaUniversità di Napoli Federico IINapoliItaly

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