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Inhibitory effect of C-reactive protein on streptolysin O-mediated hemolytic activity. Comparison of conformational variants

  • P. G. Nazarov
  • L. K. Berestovaya
Pharmacology and Toxicology

Abstract

It is shown that C-reactive protein binds to streptolysin O, an exotoxin of hemolytic streptococcus, and neutralizes its hemolytic activity. Incubation of C-reactive protein with the working dose of streptolysin O for 15–20 min at 37°C abolished the hemolysis of subsequently added erythrocytes. The concentration of C-reactive protein that reduced hemolysis by 50% was on average equal to 2.28±0.19 μg/ml. C-reactive protein antihemolytic activity was not affected by blocking of its phosphorylcholine-specific sites with free phosphorylcholine, but decreased as a result of blocking with pneumococcal C-polysaccharide and, particularly, with L-α-phosphatidylcholine. This indicates a hydrophobic nature of C-reactive protein-streptolysin O interaction. C-reactive protein subunits retained antihemolytic activity, while the aggregated C-reactive protein lost part of it.

Key Words

C-reactive protein streptolysin O erythrocytes 

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • P. G. Nazarov
    • 1
    • 2
  • L. K. Berestovaya
    • 1
    • 2
  1. 1.Institute of Experimental MedicineRussian Academy of Medical SciencesSt. Petersburg
  2. 2.Institute of vaccines and AntiseraMinistry of Health and Medical Industry of RussiaSt. Petersburg

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