Selective gas chromatographic determination of cholesterol in eggs

  • F. Guardiola
  • R. Codony
  • M. Rafecas
  • J. Boatella
Article

Abstract

A selective gas-chromatographic method is proposed for the determination of cholesterol in egg samples. The method consists of extraction of the lipid fraction and cold saponification prior to determination on a glass capillary column. The absence of cholesterol oxidation during the process was checked, and the conditions in which oxidation is avoided are discussed. The method also showed good precision (coefficient of variation=1.39%). Applying this method to different fresh and frozen egg samples, we obtained a mean content of 392 mg of cholesterol/100 g of edible portion. These values are clearly lower than the majority of results published for eggs.

Key Words

Cholesterol cholesterol oxidation eggs gas-chromatographic determination 

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References

  1. 1.
    World Heath Organization,Prevention of Coronary Heart Disease, World Health Organization Technical Report Series, 678, World Health Organization, Geneve, 1982.Google Scholar
  2. 2.
    National Institute of Health,J.A.M.A. 253:2080 (1985).Google Scholar
  3. 3.
    European Atherosclerosis Society,Eur. Heart J. 8:77 (1987).Google Scholar
  4. 4.
    López, S.H., W.A. Krehl, R.E. Hodges and E.L. Good,Am. J. Clin. Nutr. 19:361 (1966).Google Scholar
  5. 5.
    Masironi, F.,Bull. WHO 42:103 (1970).Google Scholar
  6. 6.
    Stamler, J., R. Stamler and R.B. Shekelle, inIschemic Heart Disease, edited by J.H. de Haas, H.C. Hemker and H.A. Snellen, Leiden University Press, Leiden, 1970, p. 84.Google Scholar
  7. 7.
    Armstrong, B.K., J.I. Mann, A.M. Adelstein and F. Eskin,J. Chron. Dis. 28:455 (1975).CrossRefGoogle Scholar
  8. 8.
    Keys, A., A. Menotti, M.J. Karvonen and C. Aravanis,Am. J. Epidemiol. 124:903 (1986).Google Scholar
  9. 9.
    Pyörälä, K.,Am. J. Clin. Nutr. 45:1176 (1987).Google Scholar
  10. 10.
    McNamara, D.J.,Current Opinion Lipidology 1:18 (1990).CrossRefGoogle Scholar
  11. 11.
    Ulbricht, T.L.V., and D.A.T. Southgate,Lancet 338:985 (1991).CrossRefGoogle Scholar
  12. 12.
    Kestin, M., P.M. Clifton, I.L. Rouse and P.J. Nestel,Am. J. Clin. Nutr. 50:528 (1989).Google Scholar
  13. 13.
    Edington, J.D., M. Geekie and R. Carter,Br. Med. J. 294:333 (1987).CrossRefGoogle Scholar
  14. 14.
    Schonfeld, G., W. Patsch, L.L. Ridel, C. Nelson, M. Epstein and R.E. Olson,J. Clin. Invest. 69:1072 (1982).CrossRefGoogle Scholar
  15. 15.
    Berner, Y., M. Bar-El, B. Habot and M. Shinitzky, inQuality of Poultry Products II. Eggs and Egg Products, edited by A. Oosterwoud, and A.W. de Vries, Proceedings of the 4th European Symposium on the Quality of Eggs and Egg Products, Spelderholt Center for Poultry Research and Information Services, Beekbergen, Holanda 1991, p. 91.Google Scholar
  16. 16.
    Organization for Economic Cooperation and Development,Food Consumption Statistics 1976–1985, Publications Service of Organizations for Economic Cooperation and Development, Paris, 1988.Google Scholar
  17. 17.
    Richardson, D.I.S.,Int. Eng Market Rev. 49:1, 26 (1992).Google Scholar
  18. 18.
    Beyer, R.S., and L.S. Jensen,J. Agr. Food Chem. 37:917 (1989).CrossRefGoogle Scholar
  19. 19.
    Ulberth, F., and H. Reich,Food Chem. 43:387 (1992).CrossRefGoogle Scholar
  20. 20.
    Marshall, M.W., B.A. Clevidence, R.H. Thompson and J.T. Judd,J. Food Comp. Anal. 2:2 (1989).CrossRefGoogle Scholar
  21. 21.
    Folch, J., M. Lees and G.H. Sloane-Stanley,J. Biol. Chem. 226:497 (1957).Google Scholar
  22. 22.
    Bascoul, J., N. Domergue, M. Olle and A. Crastes de Paulet,Lipids 21:383 (1986).CrossRefGoogle Scholar
  23. 23.
    Maerker, G., and J. Unruh,J. Am. Oil Chem. Soc. 63:767 (1986).Google Scholar
  24. 24.
    Naber, E.C., and M.D. Bigger,Poult. Sci. 64:341 (1985).Google Scholar
  25. 25.
    Pie, J.E., K. Spahis and C. Seillan,J. Agric. Food Chem. 38:973 (1990).CrossRefGoogle Scholar
  26. 26.
    Pie, J.E., and C. Seillan,Lipids 27:270 (1992).CrossRefGoogle Scholar
  27. 27.
    Thompson, R.H., and G.V. Merola,JAOAC 76:1057 (1993).Google Scholar
  28. 28.
    Littmann-Nienstedt, S.,Deutsche Lebensmittel-Rundschau 89:283 (1993).Google Scholar
  29. 29.
    Park, S.W., and P.B. Addis,Anal. Biochem. 149:245 (1985).CrossRefGoogle Scholar
  30. 30.
    Bohac, C.E., K.S. Rhee, H.R. Cross and K. Ono,J. Food Sci. 53:1642 (1988).CrossRefGoogle Scholar
  31. 31.
    Knoll, J.E.,J. Chromatogr. Sci. 23:422 (1985).Google Scholar
  32. 32.
    Scherz, H., and F. Senser,Souci, Fachman And Kraut Food Composition and Nutrition Tables, Wissenschaftliche Verlagsgesellschaft GmbH, Stuttgart, 1989, pp. 124–126.Google Scholar

Copyright information

© AOCS Press 1994

Authors and Affiliations

  • F. Guardiola
    • 1
  • R. Codony
    • 1
  • M. Rafecas
    • 1
  • J. Boatella
    • 1
  1. 1.Nutrition and Food Science Unit, Faculty of PharmacyUniversity of BarcelonaBarcelonaSpain

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