Letters in Peptide Science

, Volume 9, Issue 4–5, pp 203–206 | Cite as

An accurate method for the quantitation of Fmoc-derivatized solid phase supports

Article

Summary

By performing the Fmoc resin loading determination with DBU instead of piperidine, highly reproducible results were obtained that showed excellent correlation with data obtained by independent analytical methods.

Key words

Fmoc quantitation loading determination resin substitution 

Abbreviations

ACN

acetonitrile

Ams

O-amino-L-serine

BHA

benzhydrylamine functionalised polystyrene

Boc

t-butoxycarbonyl

DBF

dibenzofulvene

DBU

1,8-diazobicyclo[5.4.0]undec-7-ene

DCM

dichloromethane

DMF

dimethylformamide

Fmoc

fluoren-9-ylmethoxycarbonyl

HMPB

4-(4-hydroxymethyl-4-methoxyphenoxy)-butyryl

Me

methyl

Ph

phenyl

SPPS

solid phase peptide synthesis

TFA

trifluoroacetic acid

Trt

trityl

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References

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Calbiochem-Novabiochem AGLäufelfingenSwitzerland
  2. 2.CN Biosciences (U.K.) LtdBeestonEngland

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